Detailed information NTG0561546

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: L3475_RS08070
• Genome accession: NZ_CP091451
• Coordinates: 1588857..1591253 (-)
• Length: 798 a.a.
• NCBI ID: WP_001152098.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain 3238/09
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1583857..1596253

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L3475_RS08050 (L3475_07985)	stkP	1583872..1585851 (-)	1980	WP_000614538.1	serine/threonine-protein kinase StkP	Regulator
L3475_RS08055 (L3475_07990)	-	1585848..1586588 (-)	741	WP_000406247.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
L3475_RS08060 (L3475_07995)	rsmB	1586603..1587916 (-)	1314	WP_001814742.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
L3475_RS08065 (L3475_08000)	fmt	1587909..1588844 (-)	936	WP_000163693.1	methionyl-tRNA formyltransferase	-
L3475_RS08070 (L3475_08005)	priA	1588857..1591253 (-)	2397	WP_001152098.1	primosomal protein N'	Machinery gene
L3475_RS08075 (L3475_08010)	rpoZ	1591319..1591633 (-)	315	WP_000979235.1	DNA-directed RNA polymerase subunit omega	-
L3475_RS08080 (L3475_08015)	gmk	1591658..1592284 (-)	627	WP_000775044.1	guanylate kinase	-
L3475_RS08085 (L3475_08020)	-	1592414..1594027 (-)	1614	WP_000404930.1	ribonuclease Y	-
L3475_RS08090 (L3475_08025)	-	1594296..1594526 (-)	231	WP_000388573.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
L3475_RS08095 (L3475_08030)	-	1594764..1595861 (-)	1098	WP_000156347.1	nucleotidyltransferase	-

Sequence

Protein

• Length: 798 a.a.
• Molecular weight: 90055.82 Da
• Isoelectric Point: 6.1576

>NTDB_id=561546 L3475_RS08070 WP_001152098.1 1588857..1591253(-) (priA) [Streptococcus pneumoniae strain 3238/09]
MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPV
LTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
GLLVLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIV
QKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEIS
LTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYH
AREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQ
DRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQ
KAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSE
RTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKR
AYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL

Nucleotide

• Length: 2397 bp

>NTDB_id=561546 L3475_RS08070 WP_001152098.1 1588857..1591253(-) (priA) [Streptococcus pneumoniae strain 3238/09]
ATGGCCCTAGCTAAGATTATCGTAGATGTGCCCTTGATGCAGACGGACCAGCCCTATAGTTACAGGATTCCGGAGGAATT
TGAGGGAATGCTGGAAGTTGGGATGCGGGTTCATGTGCCTTTTGGTAAGGGTAATCGCCTGATTCAAGGGATTGTTCTTG
GTTTGGAGTCCCAATCAGATGGAGAAGAGATGGAGCAAGATTTAAAAGATATTGCAGAGGTGCTGGATTTTTCTCCTGTT
CTCACGCCAGAACAACTCTGGCTGGCTGAGGAGTTACGCAAATCTGTCTTCTCCTACAAAATTTCTATCCTCAAGGCCAT
GCTTCCAGGATTTCTGAATTCTAGCTATGACAAGATTCTCTATCCTCTGGAAGGCTTGAGTCAGGAAGAACGAGTGCGCC
TGTTTGGTTCAGAAGATTCGCTAGCCTTTTCTTCACTAGACCTTGCCAAGCAAGCTGAAATGATGCGTTTGACTAGAAAA
GGCCTGCTTGTTCTGGAATATCAGGCAGTCGATCAAAAGAAGGTCAAGACCCAGTCTTGGTATGAGGTTGACCATGCTCA
ATTAGAAGGTGTTGAGATTTCTACACGTGCCAAGAAAAAGTTGGAACTGAGAGATTATCTGCTGTCTCATCCAGAGAGCG
CTTCCTTGGCTAGCTTGTTAGAGTCCTACTCGCGAGAGCAAGTCAACTTCTTTGTGGATCAAGGTGCTGTTACCATAGTC
CAAAAGGAAGTTCAACGCTCGGCTGCTTATTTTGAAGGAATTGAAGCAAGTAGACCTTTGGAGTTAAATCCAGAACAAAG
ACAGGCGCGTGATGCGGTTGTGAGTTCTATTGGCAGTTCTCAACCTCCCTTTCTACTTCAAGGGATTACAGGAAGTGGGA
AGACCGAGGTTTACTTGCAGATTATCCAAGGTGCCCTGGATAAGGGCAAGACAGCTATTTTGCTGGTACCTGAGATTTCC
TTGACTCCTCAGATGACCGAGCGTTTTATAGCACGTTTTGGGGACAAGGTAGCCATTCTTCACTCAGGATTATCCAATGG
TGAAAAGTATGATGAATGGCGCAAGGTGGAGCGTGGCGATGCCCAAGTTGTTGTTGGTGCCAGATCTGCTATCTTTGCTC
CTCTGAAAAATCTGGGTGTCATGATTATTGATGAAGAGCATGAAGCGGCTTATAAGCAGGACAGCAATCCCCGTTACCAT
GCCAGAGAGGTAGCTATTTTACGGGCTCAGTATAATCAAGCAACTCTGGTACTTGGATCTGCAACGCCGAGCTTAGAGAG
CCGGGCACGTGCTGGCAAAGGCGTCTATCAACACTTACGTCTAACCCAACGTGCCAATCCTTTGGCTACAATCCCTGAGG
TTCAAGTGATTGACTTTCGAGACTATATCGGACAAAATGAGACGTCAAACTTTACGCCTCCTTTGCTAGAGGCTATCCAA
GACCGTCTGGTTAAAAAAGAGCAGGTGGTTCTCATGCTCAATCGCCGTGGTTATTCTAGCTTTGTCATGTGTCGGGAGTG
TGGGACGGTGGATACTTGTCCCAACTGCGATATTTCTCTGACCTTGCATATGGATACCAAGACTATGAACTGCCATTATT
GTGGTTTCTCGAAGGATATTCCTCAGGTCTGTCCTAACTGTAAGAGCCGCAGTATTCGTTACTATGGGACGGGAACTCAG
AAGGCTTATGATGAGCTAGCAGAACTCTTTCCCCAAGCTCGCATTTTGAGGATGGATGTGGATACGACTCGAAAGAAAGG
CAGTCACCAAGCCTTGCTTGACCAGTTTGGGCGAGGGGAAGCGGATATTTTACTTGGTACTCAGATGATTGCAAAGGGTT
TGGATTTTCCCAATGTTACCCTAGTCGGAGTTCTAAATGCGGATACGGCCTTGAATCTGCCTGATTTCCGTTCTTCTGAG
AGAACCTTCCAGCTCTTGACTCAGGTGGCAGGTCGAGCAGGTCGGGCTGAAAAAGCAGGTCAAGTCTTGATACAGTCCTA
CAATTCGCAGCACTATGCTATTCGATTTGCCAAGGATCAAGATTACGAAGGCTTTTATGCCTATGAAATGGGAATCAGAC
GACAACTCGGCTATCCACCTTACTATTTCACGATTGGTATTACCCTTTCTCACAAGAAAGAAGAAGAGGTTTTCAAACGT
GCCTATGAAGTCATGAACATTTTGCGGTCAGGCTTGTCAGAGACCAGTCCCATTCTGGGGCCAACGCCAAAACCGATTGC
CCGTACCCACAACCTCTATCATTACCAGATTTTAATTAAATACCGTTTAGAAGATGAGCTGGGGCCGACCCTCAACCAGG
TCTTGGCCTTGACTCAAGAACGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCGCAGCAATTTTTATAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (260-424)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-109)

• Primosomal protein N C-terminal domain (PriA_C) (700-794)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (513-538)

• Helicase conserved C-terminal domain (Helicase_C) (572-656)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	46.059	100	0.469