Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   KEF49_RS12685 Genome accession   NZ_CP073635
Coordinates   2520928..2523285 (-) Length   785 a.a.
NCBI ID   WP_115997693.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2515928..2528285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS12665 (KEF49_12665) - 2516012..2517565 (+) 1554 WP_115997692.1 SH3 domain-containing protein -
  KEF49_RS12670 (KEF49_12670) dtd 2517592..2518035 (-) 444 WP_013353029.1 D-aminoacyl-tRNA deacylase -
  KEF49_RS12675 (KEF49_12675) relA 2518048..2520252 (-) 2205 WP_065981967.1 GTP diphosphokinase -
  KEF49_RS12680 (KEF49_12680) - 2520410..2520922 (-) 513 WP_013353031.1 adenine phosphoribosyltransferase -
  KEF49_RS12685 (KEF49_12685) recJ 2520928..2523285 (-) 2358 WP_115997693.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  KEF49_RS12690 (KEF49_12690) - 2523341..2523667 (-) 327 WP_115997694.1 lipopolysaccharide assembly LapA domain-containing protein -
  KEF49_RS12695 (KEF49_12695) - 2523731..2524228 (-) 498 WP_115997695.1 cation:proton antiporter regulatory subunit -
  KEF49_RS12700 (KEF49_12700) secDF 2524359..2526578 (-) 2220 WP_115997696.1 protein translocase subunit SecDF -
  KEF49_RS12705 (KEF49_12705) - 2526612..2526911 (-) 300 WP_115997697.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87339.74 Da        Isoelectric Point: 4.9545

>NTDB_id=559605 KEF49_RS12685 WP_115997693.1 2520928..2523285(-) (recJ) [Bacillus amyloliquefaciens strain Ba13]
MLASKMRWEIQQPDKEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKLAVSRG
EPIMIYGDYDADGVTSTSVMLTALRQLSANVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCSYPFKELAGVGVAFKLAHALTGSLPEAMLDLAAIGTVADLVPLHDENRLI
ASLGIERLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTDDQFEAEELASDIDRLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVAKAGWNPGVVGIVASKLAERFYRPAVVLGIDEEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVADLRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVESITELNMLSPFGMLN
PKPHVMVENAVLDEVRLIGANKNHVKMTVKNDSAVLDCVGFNKGELEAGIVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVTALPKEKRVLISFNEKTAQKLRAELSGELAEITSESEAEALPLDGKYAVFLDVPPSADRL
QRMLDGKRPERIYFVFINHEEHFLSSFPSRNDFKWFYAFLLKRGTFDLGRHGSELARHRGWSIDTINFMTKVFFDLGFVK
IEDGVLSVVSGAKKRDLADSQTYQVKQQLMELDQKLNYSAAHELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=559605 KEF49_RS12685 WP_115997693.1 2520928..2523285(-) (recJ) [Bacillus amyloliquefaciens strain Ba13]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATAAGGAAAAGGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCGCTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGTGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAGCTGGCGGTTTCCCGCGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCAGTGATGCTGACGGCGCTCAGACAGCT
GTCAGCGAACGTTGACTTTTATATTCCCGACCGTTTCACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGCTCCATAA
AAGAGCGGGGATTTTCACTTATCATTACAGTGGATACGGGTATCGCCGCCGTTCATGAAGCTGAAGTCGCAAAAGAGCTC
GGTTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATTATCCACCCGAAGCA
GCCGGGCTGCAGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTAGCGTTTAAGCTTGCCCACGCGCTTACGGGTTCTT
TGCCGGAAGCTATGCTGGACTTGGCGGCGATCGGTACGGTTGCTGATCTGGTGCCCCTGCATGATGAAAACAGATTAATA
GCGTCGCTTGGGATCGAAAGGCTTCGGACGACAAATCGGCCGGGTCTCAAAGCTCTGATTAAGCTTTCCGGCGGCGATAT
CGGACAAGCTGATGAGGAAACGATCGGGTTTCAGGTCGCACCGAGGCTGAATGCCGTCGGAAGAATAGAACAGGCTGATC
CCGCCGTGCACCTGTTGATGACCGATGACCAGTTTGAAGCGGAGGAGCTGGCTTCGGATATTGACCGGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTTCAGCAGCAAGGCGGAAACCCTTCTGCCATTGT
CGTGGCAAAAGCAGGCTGGAATCCGGGGGTTGTCGGTATTGTCGCATCGAAGCTGGCTGAACGCTTTTACCGGCCCGCTG
TCGTGCTGGGGATTGATGAGGAGCAGGGAATCGCCAAAGGGTCGGCGCGAAGCATCAGAGGCTTTAACCTTTTTACGAGC
CTATCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGACATCCGATGGCCGCAGGAATGACTTTGAAGGCTGAAGATGT
GGCGGATTTAAGGGACCGGCTGAATCAAATGGCTGAAGAAACCCTTACCGAAGAGGATTTTATCCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTCGAAGACATCACCGTTGAAAGCATTACAGAATTGAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCTCATGTGATGGTGGAAAACGCCGTGCTTGATGAAGTGCGGCTGATCGGAGCAAATAAAAATCACGTGAAAAT
GACGGTGAAAAATGATTCCGCCGTTCTCGACTGCGTCGGCTTTAACAAAGGAGAGCTTGAAGCCGGGATTGTTCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCTATTAATGAATGGAACAACAGAAAAAAACCCCAGCTGATGATTAAAGATGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATCAGGGGCAAGCGGACGTGGGAAGACACGGTCACTGCGCTGCCTAAGGAAAA
ACGGGTGCTCATCAGCTTTAACGAAAAAACCGCCCAAAAGCTCCGTGCCGAGCTTTCGGGTGAGCTGGCTGAAATCACGT
CGGAATCCGAAGCGGAAGCTCTTCCGCTTGACGGAAAGTATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGACCGCCTC
CAGCGTATGCTTGACGGGAAGAGGCCGGAACGGATTTATTTTGTTTTTATTAATCATGAAGAACATTTCCTGTCCTCATT
TCCATCGCGGAATGACTTCAAGTGGTTTTACGCATTTCTCTTGAAAAGGGGCACTTTTGACTTAGGCAGGCATGGTTCAG
AGCTTGCAAGACACAGAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTTAAA
ATAGAAGATGGTGTGCTGTCTGTCGTGAGCGGCGCGAAAAAACGCGACCTCGCAGATTCGCAAACCTATCAGGTAAAACA
GCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCAGCCCATGAGCTGAAGGAATGGCTGAATAAGCTGATGAACC
AAGGTTCAGAGGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(315-437)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-768)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

81.934

100

0.82