Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   J8N01_RS05250 Genome accession   NZ_CP073301
Coordinates   640163..642505 (+) Length   780 a.a.
NCBI ID   WP_013059310.1    Uniprot ID   A0A7W3NCD2
Organism   Priestia megaterium strain HT517     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 635163..647505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8N01_RS05235 (J8N01_05170) spoVB 635680..637230 (-) 1551 WP_013059313.1 stage V sporulation protein B -
  J8N01_RS05240 (J8N01_05175) - 637354..637647 (+) 294 WP_013059312.1 post-transcriptional regulator -
  J8N01_RS05245 (J8N01_05180) secDF 637785..640022 (+) 2238 WP_013059311.1 protein translocase subunit SecDF -
  J8N01_RS05250 (J8N01_05185) recJ 640163..642505 (+) 2343 WP_013059310.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  J8N01_RS05255 (J8N01_05190) - 642521..643033 (+) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  J8N01_RS05260 (J8N01_05195) - 643219..645417 (+) 2199 WP_013059308.1 RelA/SpoT family protein -
  J8N01_RS05265 (J8N01_05200) dtd 645433..645894 (+) 462 WP_013059307.1 D-aminoacyl-tRNA deacylase -
  J8N01_RS05270 (J8N01_05205) - 645885..646769 (-) 885 WP_235565691.1 N-acetylmuramoyl-L-alanine amidase -
  J8N01_RS05275 (J8N01_05210) - 646936..647112 (+) 177 WP_013059305.1 hypothetical protein -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87902.49 Da        Isoelectric Point: 4.7506

>NTDB_id=558899 J8N01_RS05250 WP_013059310.1 640163..642505(+) (recJ) [Priestia megaterium strain HT517]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKEAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVESMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMHKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQDEEQAKACMFAGEQIVLLDLPPSR
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=558899 J8N01_RS05250 WP_013059310.1 640163..642505(+) (recJ) [Priestia megaterium strain HT517]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGCATTCAGCAGGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCTGTTATGCTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGAGGCATTTGATAAAATAA
GCGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGCACGTGAATTA
AACTTTGATTTAATTATTACTGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTAATTGGGACGATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGATTATCTCAAGCTCACGAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATTGGATTTGCGATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCCGATC
CAGCTGTTCATTTACTTATGACGACAAATGCTGAAGAGGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAAGAAGCCGTTGAATTTGTTGAGAGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAATTCTATAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGTTTTGACTTATTC
GCGAACTTATCTAAGTGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCACAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCATGCTTCAACAATTGATAAATATAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTCACGG
AAATGGCTTGAATGTTTGTTTGTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(305-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7W3NCD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.769

100

0.558