Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   L1P13_RS01395 Genome accession   NZ_CP091024
Coordinates   304380..305144 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain STEC379     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 299380..310144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1P13_RS01370 yhhJ 301236..302360 (+) 1125 WP_001314210.1 ABC transporter permease -
  L1P13_RS01375 - 302464..302694 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  L1P13_RS01380 - 302691..303050 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  L1P13_RS01385 nikR 303170..303571 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  L1P13_RS01390 nikE 303577..304383 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  L1P13_RS01395 amiE 304380..305144 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  L1P13_RS01400 nikC 305144..305977 (-) 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
  L1P13_RS01405 nikB 305974..306918 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  L1P13_RS01410 nikA 306918..308492 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  L1P13_RS01415 acpT 308603..309190 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=557599 L1P13_RS01395 WP_001136236.1 304380..305144(-) (amiE) [Escherichia coli strain STEC379]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=557599 L1P13_RS01395 WP_001136236.1 304380..305144(-) (amiE) [Escherichia coli strain STEC379]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398