Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KAF59_RS01310 Genome accession   NZ_CP072849
Coordinates   278396..278986 (+) Length   196 a.a.
NCBI ID   WP_000157690.1    Uniprot ID   A0A366AJW1
Organism   Vibrio cholerae MO10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273396..283986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAF59_RS01295 (KAF59_01295) - 274193..276670 (-) 2478 WP_000668733.1 penicillin-binding protein 1A -
  KAF59_RS01300 (KAF59_01300) pilM 276813..277829 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  KAF59_RS01305 (KAF59_01305) pilN 277819..278403 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  KAF59_RS01310 (KAF59_01310) pilO 278396..278986 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  KAF59_RS01315 (KAF59_01315) pilP 278976..279494 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  KAF59_RS01320 (KAF59_01320) pilQ 279520..281235 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ family protein Machinery gene
  KAF59_RS01325 (KAF59_01325) aroK 281422..281946 (+) 525 WP_000818616.1 shikimate kinase AroK -
  KAF59_RS01330 (KAF59_01330) aroB 281974..283059 (+) 1086 WP_000439805.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22877.27 Da        Isoelectric Point: 4.4169

>NTDB_id=556301 KAF59_RS01310 WP_000157690.1 278396..278986(+) (pilO) [Vibrio cholerae MO10]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=556301 KAF59_RS01310 WP_000157690.1 278396..278986(+) (pilO) [Vibrio cholerae MO10]
ATGGCTAGTCTACAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGTTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCGACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCTCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGGAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGTGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366AJW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

100

100

1
  pilO Vibrio campbellii strain DS40M4

71.958

96.429

0.694