Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   J8Y16_RS05795 Genome accession   NZ_CP072766
Coordinates   1110139..1113654 (+) Length   1171 a.a.
NCBI ID   WP_212804912.1    Uniprot ID   -
Organism   Bacillus cereus strain BC06     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1105139..1118654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8Y16_RS05760 (J8Y16_05760) - 1105471..1106248 (-) 778 Protein_1043 phosphotransferase -
  J8Y16_RS05765 (J8Y16_05765) cspA 1106600..1106803 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  J8Y16_RS05770 (J8Y16_05770) - 1106991..1107203 (-) 213 WP_062923333.1 hypothetical protein -
  J8Y16_RS05775 (J8Y16_05775) - 1107487..1107672 (-) 186 WP_000391382.1 hypothetical protein -
  J8Y16_RS05780 (J8Y16_05780) - 1107954..1108535 (+) 582 WP_000428957.1 competence protein ComK -
  J8Y16_RS05785 (J8Y16_05785) - 1108809..1109402 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  J8Y16_RS05790 (J8Y16_05790) lepB 1109459..1110022 (+) 564 WP_212804911.1 signal peptidase I -
  J8Y16_RS05795 (J8Y16_05795) addB 1110139..1113654 (+) 3516 WP_212804912.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  J8Y16_RS05800 (J8Y16_05800) addA 1113651..1117376 (+) 3726 WP_212804913.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  J8Y16_RS05805 (J8Y16_05805) - 1117392..1117679 (+) 288 WP_000255719.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  J8Y16_RS05810 (J8Y16_05810) gerPF 1117717..1117932 (-) 216 WP_001141566.1 spore germination protein GerPF -
  J8Y16_RS05815 (J8Y16_05815) - 1117975..1118361 (-) 387 WP_088119289.1 spore germination protein GerPE -
  J8Y16_RS05820 (J8Y16_05820) gerPD 1118377..1118571 (-) 195 WP_001052802.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134345.55 Da        Isoelectric Point: 5.3742

>NTDB_id=555395 J8Y16_RS05795 WP_212804912.1 1110139..1113654(+) (addB) [Bacillus cereus strain BC06]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSSALAHLEMHYEARPNEKFHGEASVTIHTAANL
RAEVEGVAREIRRLVAEENYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSTVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKSLDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVIRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFKNIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=555395 J8Y16_RS05795 WP_212804912.1 1110139..1113654(+) (addB) [Bacillus cereus strain BC06]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAGGAAGAGTTAAA
ACAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGCA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTTCATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAACAAAACGGCTTCTTTGAACATCTCGGCAGTATGATTGCAGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCAGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTTGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTCGAAAAGCTTCCGCAATCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGATTTCACTCAT
TTTCACCGCAAGAGTTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACAATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAATTAGATTTATTTTATGAAACGACATTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCTTCGGCATTAGCGCATT
TAGAAATGCATTACGAAGCGCGTCCAAATGAAAAGTTTCACGGTGAAGCAAGTGTAACGATTCATACAGCAGCTAATTTA
CGAGCGGAAGTAGAAGGCGTTGCTCGTGAAATTCGTAGACTTGTGGCTGAAGAAAACTATCGTTACCGAGATATTGCGGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTAATGCGGACACTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCACTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTATTGCTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGTCGCT
ATCGTTCTCTTGACGATACGAACGGGATGATCACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGCGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTGGACGTTCCAAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGTGGGGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTGATTGCTAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTTATTGGCGTGAATGAAGGTG
TCATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGATGTTTTGAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCCGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCG
GAAGTAACACTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTAACTTCGGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ACCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATACGGAGACAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTTGATGC
ACCAGATATCGGAGAGCTCTTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTACTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGGCAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCCATTATTCTACGTGAACA
TGCGAAGTCGAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTTGGTATGGGCGGTACAGGATCACTTCCGCCGATGG
AATTCTCGTTACCAAATGGTGTAAAGATGGAAGTAGTCGGCCGTATTGACCGCGTTGATAAGGCGGAAGATGAAAACGGA
ACATTCCTCCGTATTATTGACTATAAATCAAGCTCAAAATCGTTAGACTTAACGGAAGTGTATTACGGATTGGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACAGACGTGGATGAAAAAAGGCCACGCAGCATCACCAGCTGGTG
TACTGTACTTCCACATTCATAACCCAATTGTTGAGGTGAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGGCTCGTACTAGGAGATGCTGACGTTATTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCAAGTATTGCCAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACATCATACGTTTAAAAATATCGGAAAAGACATTACAGAGGGTGTTATCGATATTGCTCCA
TACAAAAAGGGGAATAAAGCAGCGTGTACGTTCTGTAACTTCAAATCAGTTTGTCAGTTCGATGAATCACTTGAAGATAA
TCAATTCCGTACGCTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.061

100

0.491