Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   LSG45_RS24240 Genome accession   NZ_CP089238
Coordinates   5228185..5228610 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain JNQH-PA033     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5223185..5233610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSG45_RS24225 (LSG45_24225) pilX 5223749..5224336 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  LSG45_RS24230 (LSG45_24230) pilY1 5224348..5227839 (+) 3492 WP_023111845.1 type 4a pilus biogenesis protein PilY1 -
  LSG45_RS24235 (LSG45_24235) pilY2 5227841..5228188 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  LSG45_RS24240 (LSG45_24240) comF 5228185..5228610 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  LSG45_RS24245 (LSG45_24245) ispH 5228657..5229601 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  LSG45_RS24250 (LSG45_24250) fkpB 5229687..5230127 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  LSG45_RS24255 (LSG45_24255) lspA 5230120..5230629 (-) 510 WP_003110420.1 signal peptidase II -
  LSG45_RS24260 (LSG45_24260) ileS 5230622..5233453 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=549828 LSG45_RS24240 WP_003094721.1 5228185..5228610(+) (comF) [Pseudomonas aeruginosa strain JNQH-PA033]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=549828 LSG45_RS24240 WP_003094721.1 5228185..5228610(+) (comF) [Pseudomonas aeruginosa strain JNQH-PA033]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCACAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACACCCACCAGCACTACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383