Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   J4048_RS12750 Genome accession   NZ_CP071970
Coordinates   2640294..2641331 (-) Length   345 a.a.
NCBI ID   WP_015417819.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain XJ5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2635294..2646331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4048_RS12700 (J4048_12700) - 2635528..2636313 (-) 786 WP_007408329.1 TasA family protein -
  J4048_RS12705 (J4048_12705) - 2636378..2636962 (-) 585 WP_015240205.1 signal peptidase I -
  J4048_RS12710 (J4048_12710) tapA 2636934..2637605 (-) 672 WP_031378945.1 amyloid fiber anchoring/assembly protein TapA -
  J4048_RS12715 (J4048_12715) - 2637864..2638193 (+) 330 WP_039254490.1 DUF3889 domain-containing protein -
  J4048_RS12720 (J4048_12720) - 2638233..2638412 (-) 180 WP_003153093.1 YqzE family protein -
  J4048_RS12725 (J4048_12725) comGG 2638469..2638846 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  J4048_RS12730 (J4048_12730) comGF 2638847..2639347 (-) 501 WP_257738552.1 competence type IV pilus minor pilin ComGF -
  J4048_RS12735 (J4048_12735) comGE 2639256..2639570 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  J4048_RS12740 (J4048_12740) comGD 2639554..2639991 (-) 438 WP_015417817.1 competence type IV pilus minor pilin ComGD Machinery gene
  J4048_RS12745 (J4048_12745) comGC 2639981..2640289 (-) 309 WP_015417818.1 competence type IV pilus major pilin ComGC Machinery gene
  J4048_RS12750 (J4048_12750) comGB 2640294..2641331 (-) 1038 WP_015417819.1 competence type IV pilus assembly protein ComGB Machinery gene
  J4048_RS12755 (J4048_12755) comGA 2641318..2642388 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  J4048_RS12760 (J4048_12760) - 2642581..2643531 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  J4048_RS12765 (J4048_12765) - 2643677..2644978 (+) 1302 WP_012117986.1 hemolysin family protein -
  J4048_RS12770 (J4048_12770) - 2645016..2645846 (-) 831 WP_039254482.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39689.06 Da        Isoelectric Point: 9.5935

>NTDB_id=549500 J4048_RS12750 WP_015417819.1 2640294..2641331(-) (comGB) [Bacillus amyloliquefaciens strain XJ5]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLQLQLHKRQLAELKEGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLIFSVCVMFYILQSLIIPQFSGIYQSMNMNTSGTTAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=549500 J4048_RS12750 WP_015417819.1 2640294..2641331(-) (comGB) [Bacillus amyloliquefaciens strain XJ5]
ATGAAGCGGATTAAACGAACATGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGCCTGATTGAAGGGCTCAGACTGCTGCAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGAAGGAAGGAATCC
GCCGGCTGAGAGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTTTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACGTCCGGTACGACTGCTTTCATCTTTGCGTTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTTTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAGTCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCCCTCTTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGAGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
TGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614