Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CAL6303_RS23490 Genome accession   NC_019751
Coordinates   5799770..5800876 (-) Length   368 a.a.
NCBI ID   WP_015200326.1    Uniprot ID   K9V6K1
Organism   Calothrix sp. PCC 6303     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5794770..5805876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAL6303_RS23475 (Cal6303_4812) - 5795709..5797985 (-) 2277 WP_015200323.1 type IV pilus secretin family protein -
  CAL6303_RS23480 (Cal6303_4813) - 5798208..5798996 (-) 789 WP_015200324.1 hypothetical protein -
  CAL6303_RS23485 (Cal6303_4814) - 5798993..5799763 (-) 771 WP_015200325.1 PilN domain-containing protein -
  CAL6303_RS23490 (Cal6303_4815) pilM 5799770..5800876 (-) 1107 WP_015200326.1 type IV pilus assembly protein PilM Machinery gene
  CAL6303_RS23495 (Cal6303_4816) - 5801041..5802327 (+) 1287 WP_015200327.1 ABC transporter substrate-binding protein -
  CAL6303_RS23500 (Cal6303_4817) - 5802419..5804119 (-) 1701 WP_015200328.1 serine/threonine protein kinase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40569.58 Da        Isoelectric Point: 4.5844

>NTDB_id=54950 CAL6303_RS23490 WP_015200326.1 5799770..5800876(-) (pilM) [Calothrix sp. PCC 6303]
MVKSFNNFFGKSNQGVGIELSPQRVNVARLRKQKQGLKLDSLTSVPVPEGVFTNGQIADPPAMAQIIQQALAASKIKVSK
VATAVAGRDSIVRLIPVPSELDDKELREMVLNHEAGLYLPYPREEADVDYQKLGYFVDEDGIEKVQVLLVATRKEITDTY
VRTFEQAGLQIDVLEINSFALIRTIREQLRQFSPQEAAVLVDIEFDSTEIAIVVNGVPQFSRTVPIGTLQMQTALSRAMN
LPPSRDLELLQGMTIPSTPVETNKTGQTSINPGMAAMMRVLGELTDELRRSIDFYLNQSENLEVAQILLAGPGGGMIQLD
EFFTQRLSLPTTQIDPVGSLALQIDEEKYPYTQRPGLGIVLGLGMREA

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=54950 CAL6303_RS23490 WP_015200326.1 5799770..5800876(-) (pilM) [Calothrix sp. PCC 6303]
GTGGTTAAAAGCTTCAATAATTTTTTCGGCAAATCAAATCAAGGGGTAGGCATCGAACTTTCGCCGCAACGAGTTAATGT
TGCTCGGTTACGCAAGCAAAAACAAGGCTTAAAGTTAGACTCCTTAACCTCGGTGCCAGTTCCAGAGGGAGTATTCACTA
ATGGACAAATTGCTGATCCTCCAGCAATGGCACAGATCATCCAGCAAGCACTGGCAGCAAGCAAAATTAAAGTCTCTAAA
GTTGCAACTGCGGTAGCCGGACGCGATTCGATTGTCCGACTCATCCCTGTACCTTCAGAATTAGATGACAAAGAACTGCG
AGAGATGGTGCTAAACCATGAAGCTGGGTTGTACTTACCCTATCCTCGTGAAGAAGCAGATGTAGATTACCAAAAACTTG
GATATTTCGTCGATGAAGACGGAATTGAAAAAGTTCAGGTACTCTTGGTGGCAACTCGGAAAGAAATTACCGACACCTAT
GTCCGCACTTTTGAACAGGCAGGACTGCAAATCGATGTATTAGAAATTAATAGTTTTGCATTAATTCGGACAATTCGGGA
GCAGTTGCGGCAATTTAGTCCTCAAGAGGCAGCGGTATTAGTTGATATTGAGTTTGATAGCACAGAAATTGCCATTGTTG
TTAATGGTGTACCTCAATTTTCCCGTACAGTACCCATTGGCACATTGCAAATGCAAACAGCATTGTCGCGGGCAATGAAT
CTGCCTCCCTCCCGTGATTTGGAATTACTCCAAGGAATGACAATTCCTTCAACACCAGTAGAAACCAATAAAACTGGGCA
AACATCTATAAACCCTGGGATGGCAGCAATGATGAGGGTATTAGGAGAACTCACCGATGAACTGCGTCGTTCTATAGATT
TTTACTTAAATCAAAGTGAAAACTTGGAAGTCGCACAAATTTTACTAGCAGGACCAGGTGGGGGAATGATTCAGCTGGAT
GAATTCTTTACCCAAAGATTGAGTTTACCAACTACCCAAATTGATCCTGTTGGTTCGTTGGCATTACAAATTGATGAGGA
AAAGTACCCTTACACACAACGTCCTGGTTTAGGTATAGTTCTCGGTCTGGGAATGAGGGAGGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(283-367)

Type IV pilus assembly protein PilM; (PilM_2)

(17-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9V6K1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

67.391

100

0.674

Multiple sequence alignment