Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   EXW53_RS21275 Genome accession   NZ_CP071808
Coordinates   4128678..4130999 (-) Length   773 a.a.
NCBI ID   WP_002199283.1    Uniprot ID   A0A1E8BI04
Organism   Bacillus mycoides strain JAS014     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4123678..4135999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW53_RS21255 (EXW53_21310) - 4124905..4125081 (-) 177 WP_002015300.1 hypothetical protein -
  EXW53_RS21260 (EXW53_21315) - 4125248..4125688 (-) 441 WP_070144963.1 D-tyrosyl-tRNA(Tyr) deacylase -
  EXW53_RS21265 (EXW53_21320) relA 4125706..4127889 (-) 2184 WP_215557497.1 GTP diphosphokinase -
  EXW53_RS21270 (EXW53_21325) - 4128100..4128612 (-) 513 WP_002111996.1 adenine phosphoribosyltransferase -
  EXW53_RS21275 (EXW53_21330) recJ 4128678..4130999 (-) 2322 WP_002199283.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  EXW53_RS21280 (EXW53_21335) - 4131101..4131994 (-) 894 WP_002199282.1 cation diffusion facilitator family transporter -
  EXW53_RS21285 (EXW53_21340) secDF 4132215..4134479 (-) 2265 WP_098988397.1 protein translocase subunit SecDF -
  EXW53_RS21290 (EXW53_21345) - 4134761..4135045 (-) 285 WP_002112000.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 773 a.a.        Molecular weight: 87397.83 Da        Isoelectric Point: 4.9665

>NTDB_id=548025 EXW53_RS21275 WP_002199283.1 4128678..4130999(-) (recJ) [Bacillus mycoides strain JAS014]
MLQPKTRWKEKEYNEELVSELANKLQLSPLVTSLFLGRGLNTEDKIVDFLNTADQEFHDPFLLEGMDRTVERVKKAIENG
EQILIFGDYDADGVSSTTVLYLALKELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEAFAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VQRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSLAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNTL
RKDIVKQITEEAIAEVENKFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPHKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLNMNDVDELRRRLNEQADAVLTEEDFIPITAVDAFCKVEDVTLAAIEEMQKLAPFGV
GNPKPRIAVKDADLESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVVGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKEKITMVYFSEEVLNKFSLEGYKEQLVHASEVTHLDDQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLKQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMAEKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHK

Nucleotide


Download         Length: 2322 bp        

>NTDB_id=548025 EXW53_RS21275 WP_002199283.1 4128678..4130999(-) (recJ) [Bacillus mycoides strain JAS014]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGAGGAACTAGTGAGTGAATTAGCAAATAAATTGCAACT
ATCACCTCTTGTGACTTCATTATTTCTCGGTAGAGGCTTAAATACAGAAGATAAGATTGTAGACTTTTTAAATACAGCAG
ATCAAGAATTTCATGATCCATTTTTACTTGAAGGAATGGATCGGACTGTAGAACGTGTGAAAAAGGCCATTGAAAATGGA
GAACAAATATTAATATTTGGCGATTATGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCTCTCAAAGAATT
AGGTGCAGAAGTAGAATTTTATATTCCAAATCGTTTTACTGAAGGCTATGGGCCAAATGAAGAAGCGTTTCGTTGGGCGC
ACAGTGCAGGGTTCTCACTAATTATTACTGTCGATACTGGTATCGCAGCGGTACATGAAGCGAAGGTAGCGAAGGAACTT
GGGATAGATCTTATTATTACAGATCATCATGAGCCACCACCAGAGTTACCAGAGGCATTTGCGATTATTCATCCGAAATT
AGAAGGCGGTGTTTATCCGTTTCATTATTTAGCGGGTGTTGGTGTTGCATTTAAAGTTGCACATGCACTTTTGGGCCGTG
TACCAGAGCATTTATTGGAAATTGCAGTAATCGGTACGGTAGCCGATTTAGTATCGCTTCACGGTGAAAATAGATTGTTA
GTACAGCGTGGACTGAAGCATATGCGAATGACTCGAAATATCGGTTTAAAGGCGTTATTTAAAGTTGCTAACGTTTCGCA
AAGCGAAATTACAGAAGAAAGCATCGGATTCTCACTCGCACCTCGTATTAATGCAGTTGGTCGTTTGGAAGATGCAACAC
CTGCTGTACACCTTTTATTATCAGAAGATCCAGAGGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGCTGAACACACTT
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTCGAAAATAAATTCCCGCCGGAAGAAAATAAAGT
ATTAGTCCTTGCAAAAGAAGGTTGGAATCCAGGGGTAATTGGAATTGTCGCTTCTAAGTTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATTGATCCTCATAAAGAAACAGCAAAGGGATCGGCTCGTAGTATTGCAGGATTTGATTTGTTT
GCAAACCTTTCAGATTGCCGTGAATTGTTACCGCATTTCGGAGGTCATCCGATGGCGGCGGGAATGACGCTTAATATGAA
TGATGTGGATGAATTAAGACGCAGGTTAAATGAGCAAGCAGATGCAGTTTTAACAGAAGAAGATTTTATTCCGATTACAG
CAGTGGATGCATTTTGTAAAGTAGAGGATGTAACGCTTGCCGCGATTGAGGAGATGCAAAAGTTAGCGCCATTTGGCGTG
GGAAATCCGAAACCGCGTATTGCGGTGAAAGATGCTGACTTAGAAAGTATCCGTGCAATTGGATCTGATGGTTCGCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTTGGATTTGGTGCTTATGCGAAAGAAATCTCTC
CAGTTGCGAAGGTTTCTGTAGTAGGAGAAGCGTCCATTAATGAATGGAATAACTTTAAAAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGACTGGCGTAGTATGCGTAATGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACCATGGTGTATTTTTCTGAAGAGGTATTGAATAAGTTTTCTTTAGAAGGCTATAAAGAACAGCTCGTGC
ATGCATCAGAGGTAACGCATTTAGATGATCAATATATTGTGTTACTCGATTTGCCGAAAGGAACAGATGAATTGCGTGAT
TTATTTAAAGTAGGATTTCCATCTCGAATTTACACGCTATTCTATCAAGAGAACAATCATTTATTTAGTACAGTCCCAAC
GAGGGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAAGCAATATGGGGAACAACTGT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCAGAAAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAGAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAT
AA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E8BI04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.161

100

0.533