Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   LSI69_RS02615 Genome accession   NZ_CP089032
Coordinates   543151..543618 (-) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli O157:H7 strain 6535WT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 538151..548618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSI69_RS02590 (LSI69_02590) rpsG 538292..538762 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  LSI69_RS02595 (LSI69_02595) fusA 538859..540973 (+) 2115 WP_000124700.1 elongation factor G -
  LSI69_RS02600 (LSI69_02600) tuf 541044..542228 (+) 1185 WP_000031783.1 elongation factor Tu -
  LSI69_RS02605 (LSI69_02605) bfd 542411..542605 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  LSI69_RS02610 (LSI69_02610) bfr 542678..543154 (+) 477 WP_000675516.1 bacterioferritin -
  LSI69_RS02615 (LSI69_02615) pilD 543151..543618 (-) 468 WP_000381746.1 prepilin peptidase Machinery gene
  LSI69_RS02620 (LSI69_02620) rpsJ 543997..544308 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  LSI69_RS02625 (LSI69_02625) rplC 544341..544970 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  LSI69_RS02630 (LSI69_02630) rplD 544981..545586 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  LSI69_RS02635 (LSI69_02635) rplW 545583..545885 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  LSI69_RS02640 (LSI69_02640) rplB 545903..546724 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  LSI69_RS02645 (LSI69_02645) rpsS 546741..547019 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  LSI69_RS02650 (LSI69_02650) rplV 547034..547366 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  LSI69_RS02655 (LSI69_02655) rpsC 547384..548085 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  LSI69_RS02660 (LSI69_02660) rplP 548098..548508 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=547770 LSI69_RS02615 WP_000381746.1 543151..543618(-) (pilD) [Escherichia coli O157:H7 strain 6535WT]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=547770 LSI69_RS02615 WP_000381746.1 543151..543618(-) (pilD) [Escherichia coli O157:H7 strain 6535WT]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCCTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387