Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   LRM32_RS01225 Genome accession   NZ_CP088131
Coordinates   265481..266245 (-) Length   254 a.a.
NCBI ID   WP_060618111.1    Uniprot ID   -
Organism   Escherichia coli strain 81     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 260481..271245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRM32_RS01215 (LRM32_01215) nikR 264271..264672 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  LRM32_RS01220 (LRM32_01220) nikE 264678..265484 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  LRM32_RS01225 (LRM32_01225) amiE 265481..266245 (-) 765 WP_060618111.1 nickel import ATP-binding protein NikD Regulator
  LRM32_RS01230 (LRM32_01230) nikC 266245..267078 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  LRM32_RS01235 (LRM32_01235) nikB 267075..268019 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  LRM32_RS01240 (LRM32_01240) nikA 268019..269593 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  LRM32_RS01245 (LRM32_01245) acpT 269704..270291 (-) 588 WP_000285791.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26814.37 Da        Isoelectric Point: 6.4890

>NTDB_id=547262 LRM32_RS01225 WP_060618111.1 265481..266245(-) (amiE) [Escherichia coli strain 81]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAAHVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=547262 LRM32_RS01225 WP_060618111.1 265481..266245(-) (amiE) [Escherichia coli strain 81]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCACGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398