Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   J0W33_RS06420 Genome accession   NZ_CP071319
Coordinates   1340012..1340446 (-) Length   144 a.a.
NCBI ID   WP_168405348.1    Uniprot ID   -
Organism   Acinetobacter indicus strain GXNN62X4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1336673..1360115 1340012..1340446 within 0


Gene organization within MGE regions


Location: 1336673..1360115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0W33_RS14480 (J0W33_06395) - 1336673..1337651 (+) 979 Protein_1242 esterase/lipase family protein -
  J0W33_RS06400 (J0W33_06400) rplS 1337715..1338089 (-) 375 WP_168406100.1 50S ribosomal protein L19 -
  J0W33_RS06405 (J0W33_06405) trmD 1338263..1339012 (-) 750 WP_180006579.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  J0W33_RS06410 (J0W33_06410) rimM 1339039..1339587 (-) 549 WP_016658481.1 ribosome maturation factor RimM -
  J0W33_RS06415 (J0W33_06415) rpsP 1339616..1339873 (-) 258 WP_004809941.1 30S ribosomal protein S16 -
  J0W33_RS06420 (J0W33_06420) pilE 1340012..1340446 (-) 435 WP_168405348.1 type IV pilin protein Machinery gene
  J0W33_RS14440 (J0W33_06425) - 1340440..1341075 (-) 636 WP_104473453.1 type IV pilin protein -
  J0W33_RS06430 (J0W33_06430) - 1341080..1344970 (-) 3891 WP_206896993.1 hypothetical protein -
  J0W33_RS06435 (J0W33_06435) - 1344980..1345735 (-) 756 WP_180006577.1 hypothetical protein -
  J0W33_RS06440 (J0W33_06440) - 1345746..1346771 (-) 1026 WP_373685078.1 PilW family protein -
  J0W33_RS06445 (J0W33_06445) pilV 1346747..1347250 (-) 504 WP_168408685.1 type IV pilus modification protein PilV -
  J0W33_RS06450 (J0W33_06450) - 1347247..1347783 (-) 537 WP_206896994.1 Tfp pilus assembly protein FimT/FimU -
  J0W33_RS06455 (J0W33_06455) ispH 1347947..1348897 (-) 951 WP_045795274.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  J0W33_RS06460 (J0W33_06460) gmk 1349033..1349656 (+) 624 WP_005180457.1 guanylate kinase -
  J0W33_RS06465 (J0W33_06465) rpoZ 1349733..1350014 (+) 282 WP_005013174.1 DNA-directed RNA polymerase subunit omega -
  J0W33_RS06470 (J0W33_06470) - 1350238..1352337 (+) 2100 WP_075174935.1 RelA/SpoT family protein -
  J0W33_RS06475 (J0W33_06475) - 1352409..1352792 (+) 384 WP_104513240.1 RidA family protein -
  J0W33_RS06480 (J0W33_06480) - 1352947..1353141 (+) 195 WP_034597991.1 bacterioferritin-associated ferredoxin -
  J0W33_RS06485 (J0W33_06485) bfr 1353368..1353832 (+) 465 WP_104489443.1 bacterioferritin -
  J0W33_RS06490 (J0W33_06490) - 1353944..1355584 (-) 1641 WP_206896995.1 PglL family O-oligosaccharyltransferase -
  J0W33_RS06495 (J0W33_06495) tfpZ 1355677..1356411 (-) 735 WP_206896996.1 TfpX/TfpZ family type IV pilin accessory protein -
  J0W33_RS06500 (J0W33_06500) - 1356455..1356883 (-) 429 WP_206896997.1 pilin -
  J0W33_RS06505 (J0W33_06505) - 1357042..1358235 (-) 1194 WP_206896998.1 serine hydrolase domain-containing protein -
  J0W33_RS06510 (J0W33_06510) - 1358248..1359480 (-) 1233 WP_206896999.1 sulfatase-like hydrolase/transferase -
  J0W33_RS06515 (J0W33_06515) - 1359642..1360115 (-) 474 WP_206897000.1 pilin -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15884.27 Da        Isoelectric Point: 8.9420

>NTDB_id=544946 J0W33_RS06420 WP_168405348.1 1340012..1340446(-) (pilE) [Acinetobacter indicus strain GXNN62X4]
MVRQGFTLIELMIVIAIIAILAAIAYPSYQQYKIKVNRAEAQSEMMFIAERMNAYKGMNGSFSGATIAQIYGSTVTPKQG
TALYDLSFDAALTNATRWTLIAQPKVGTLQAGNGWICLNNQSEKYWAKGEARCDLSPNSTWSDK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=544946 J0W33_RS06420 WP_168405348.1 1340012..1340446(-) (pilE) [Acinetobacter indicus strain GXNN62X4]
ATGGTAAGACAAGGGTTTACACTTATAGAACTTATGATTGTCATTGCGATTATTGCAATTTTAGCTGCTATTGCTTATCC
CTCTTATCAGCAATATAAAATTAAAGTAAATCGGGCAGAAGCACAATCAGAGATGATGTTCATTGCTGAAAGAATGAATG
CCTATAAAGGAATGAATGGTTCTTTTTCGGGGGCAACGATTGCACAAATATATGGATCAACAGTAACTCCCAAACAAGGC
ACTGCATTATATGATTTAAGCTTTGATGCTGCACTAACCAATGCAACCCGTTGGACTCTAATTGCTCAGCCTAAGGTAGG
TACGTTGCAAGCGGGTAATGGGTGGATTTGTTTAAATAATCAATCAGAGAAATATTGGGCAAAAGGCGAAGCGCGTTGTG
ACTTGTCACCAAACTCTACCTGGTCCGATAAATAA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(27-111)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Acinetobacter baumannii D1279779

53.846

99.306

0.535
  comF Acinetobacter baylyi ADP1

48.951

99.306

0.486