Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   J0W32_RS06415 Genome accession   NZ_CP071317
Coordinates   1336909..1337343 (-) Length   144 a.a.
NCBI ID   WP_104473452.1    Uniprot ID   -
Organism   Acinetobacter indicus strain GXNN15X4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1337337..1363907 1336909..1337343 flank -6


Gene organization within MGE regions


Location: 1336909..1363907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0W32_RS06415 (J0W32_06415) pilE 1336909..1337343 (-) 435 WP_104473452.1 type IV pilin protein Machinery gene
  J0W32_RS14670 (J0W32_06420) - 1337337..1337972 (-) 636 WP_180088588.1 type IV pilin protein -
  J0W32_RS06425 (J0W32_06425) - 1337977..1341828 (-) 3852 WP_228289524.1 hypothetical protein -
  J0W32_RS06430 (J0W32_06430) - 1341838..1342593 (-) 756 WP_206862893.1 hypothetical protein -
  J0W32_RS06435 (J0W32_06435) - 1342604..1343602 (-) 999 WP_206862894.1 PilW family protein -
  J0W32_RS06440 (J0W32_06440) pilV 1343604..1344107 (-) 504 WP_171431621.1 type IV pilus modification protein PilV -
  J0W32_RS06445 (J0W32_06445) - 1344104..1344640 (-) 537 WP_206862895.1 Tfp pilus assembly protein FimT/FimU -
  J0W32_RS06450 (J0W32_06450) ispH 1344804..1345754 (-) 951 WP_045795274.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  J0W32_RS06455 (J0W32_06455) gmk 1345890..1346513 (+) 624 WP_045795273.1 guanylate kinase -
  J0W32_RS06460 (J0W32_06460) rpoZ 1346590..1346871 (+) 282 WP_004809957.1 DNA-directed RNA polymerase subunit omega -
  J0W32_RS06465 (J0W32_06465) - 1347094..1349193 (+) 2100 WP_160240914.1 RelA/SpoT family protein -
  J0W32_RS06470 (J0W32_06470) - 1349265..1349648 (+) 384 WP_005180461.1 RidA family protein -
  J0W32_RS06475 (J0W32_06475) - 1349803..1349997 (+) 195 WP_034597991.1 bacterioferritin-associated ferredoxin -
  J0W32_RS06480 (J0W32_06480) bfr 1350224..1350688 (+) 465 WP_005180468.1 bacterioferritin -
  J0W32_RS06485 (J0W32_06485) - 1350800..1352440 (-) 1641 WP_206862896.1 PglL family O-oligosaccharyltransferase -
  J0W32_RS06490 (J0W32_06490) pilE 1352781..1353218 (+) 438 WP_206862897.1 pilin Machinery gene
  J0W32_RS06495 (J0W32_06495) - 1353275..1355239 (+) 1965 WP_206862898.1 hypothetical protein -
  J0W32_RS06500 (J0W32_06500) - 1355229..1356080 (+) 852 WP_206862899.1 glycosyltransferase family 2 protein -
  J0W32_RS06505 (J0W32_06505) - 1356169..1357395 (+) 1227 WP_206862900.1 lipopolysaccharide biosynthesis protein -
  J0W32_RS06510 (J0W32_06510) - 1357376..1358497 (-) 1122 WP_206862901.1 glycosyltransferase family 4 protein -
  J0W32_RS06515 (J0W32_06515) - 1358502..1359614 (-) 1113 WP_206862902.1 glycosyltransferase -
  J0W32_RS06520 (J0W32_06520) - 1359611..1360513 (-) 903 WP_206862903.1 FkbM family methyltransferase -
  J0W32_RS06525 (J0W32_06525) - 1360515..1361297 (-) 783 WP_206862904.1 class I SAM-dependent methyltransferase -
  J0W32_RS06530 (J0W32_06530) - 1361657..1362718 (-) 1062 WP_206862905.1 UDP-N-acetylglucosamine 2-epimerase -
  J0W32_RS06535 (J0W32_06535) - 1362975..1363907 (+) 933 WP_206862906.1 IS5-like element IS17 family transposase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15856.22 Da        Isoelectric Point: 8.9420

>NTDB_id=544904 J0W32_RS06415 WP_104473452.1 1336909..1337343(-) (pilE) [Acinetobacter indicus strain GXNN15X4]
MVRQGFTLIELMIVIAIIAILAAIAYPSYQQYKIKVNRAEAQSEMMFIAERMNAYKGMNGSFSGATIAQIYGSTVTPKQG
TALYDLSFDAALTNATRWTLIAQPKAGTLQAGNGWICLNNQSEKYWAKGEARCDLSPNSTWSDK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=544904 J0W32_RS06415 WP_104473452.1 1336909..1337343(-) (pilE) [Acinetobacter indicus strain GXNN15X4]
ATGGTAAGACAAGGGTTTACACTTATAGAACTTATGATTGTCATTGCGATTATTGCAATTTTAGCTGCTATTGCTTATCC
CTCTTATCAGCAATATAAAATTAAAGTAAATCGGGCAGAAGCACAATCAGAGATGATGTTCATTGCTGAAAGAATGAATG
CCTATAAAGGAATGAATGGTTCTTTTTCGGGGGCAACGATTGCACAAATATATGGATCAACAGTAACTCCCAAACAAGGC
ACTGCATTATATGATTTAAGCTTTGATGCTGCACTAACCAATGCAACCCGTTGGACTCTAATTGCTCAACCTAAGGCAGG
TACGTTACAAGCGGGCAATGGTTGGATTTGTTTAAACAATCAATCAGAGAAATATTGGGCAAAAGGCGAAGCGCGTTGTG
ACTTGTCGCCAAACTCTACCTGGTCCGATAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-26)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(27-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Acinetobacter baumannii D1279779

54.545

99.306

0.542
  comF Acinetobacter baylyi ADP1

48.951

99.306

0.486