Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   LOE88_RS13135 Genome accession   NZ_CP086662
Coordinates   2724703..2725467 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain E2     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2719703..2730467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOE88_RS13115 (LOE88_13115) acpT 2720657..2721244 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  LOE88_RS13120 (LOE88_13120) nikA 2721355..2722929 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  LOE88_RS13125 (LOE88_13125) nikB 2722929..2723873 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  LOE88_RS13130 (LOE88_13130) nikC 2723870..2724703 (+) 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
  LOE88_RS13135 (LOE88_13135) amiE 2724703..2725467 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  LOE88_RS13140 (LOE88_13140) nikE 2725464..2726270 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  LOE88_RS13145 (LOE88_13145) nikR 2726276..2726677 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  LOE88_RS13150 (LOE88_13150) - 2726797..2727156 (-) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  LOE88_RS13155 (LOE88_13155) - 2727153..2727383 (-) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  LOE88_RS13160 (LOE88_13160) yhhJ 2727487..2728611 (-) 1125 WP_001314210.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=543953 LOE88_RS13135 WP_001136236.1 2724703..2725467(+) (amiE) [Escherichia coli strain E2]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=543953 LOE88_RS13135 WP_001136236.1 2724703..2725467(+) (amiE) [Escherichia coli strain E2]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGAGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398