Detailed information NTG0541714

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: JYG31_RS05780
• Genome accession: NZ_CP070976
• Coordinates: 1145747..1149247 (+)
• Length: 1166 a.a.
• NCBI ID: WP_213418491.1
• Uniprot ID: -
• Organism: Bacillus halotolerans strain MBH1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1140747..1154247

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JYG31_RS05760 (JYG31_05760)	-	1141602..1142804 (-)	1203	WP_213418490.1	MFS transporter	-
JYG31_RS05765 (JYG31_05765)	-	1142920..1144649 (+)	1730	Protein_1080	ABC transporter substrate-binding protein	-
JYG31_RS05770 (JYG31_05770)	-	1144683..1144997 (-)	315	WP_024120836.1	four-helix bundle copper-binding protein	-
JYG31_RS05775 (JYG31_05775)	-	1145126..1145563 (-)	438	WP_095713675.1	ferritin family protein	-
JYG31_RS05780 (JYG31_05780)	addB	1145747..1149247 (+)	3501	WP_213418491.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
JYG31_RS05785 (JYG31_05785)	addA	1149234..1152938 (+)	3705	WP_024120839.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
JYG31_RS05790 (JYG31_05790)	sbcD	1153010..1154188 (+)	1179	WP_213418492.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 134754.77 Da
• Isoelectric Point: 5.3844

>NTDB_id=541714 JYG31_RS05780 WP_213418491.1 1145747..1149247(+) (addB) [Bacillus halotolerans strain MBH1]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDTGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMADSGTASEYRGERILSE
KLHDLSILYQQMEKSLEDQYLHSEDYLTLLAEHIPQAEDLKGAHVYVDGFYQFTPQEFRVLEQLMLQAEHITFSLTADKP
SHEGEPQELDLFRMTGKTYYRLHQTAKELNLDITYKELDESKRHKRAPELAHLEAQYEARPVIPFAEKQEAFTVMQAANR
RAELEGIAREIHSLVREKGYRYKDMAILARQPEDYKDMVKEVFADYEIPYFIDGKTSMLNHPLVEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNQPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDEFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGEDDI
SLDLFQQMMETGAESLTFSLIPPALDQVFVGNMDLSRIYGTSCTFMIGANDGVLPARPDENGVLSDDDREWLKTIGIELS
SGGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVIKRLEELFPQHRERLLTNEPEQVSDEEQLMYLVNKS
VAQSYTASQLRLWTREYEISDVWWSAYNALMNEPDRLESKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQVFKLEAPDIGQLFHSSLKLISDRLREQKIDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSLDWLGMKATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRLLKAEKDKTVLDWIKKEADGNEHS

Nucleotide

• Length: 3501 bp

>NTDB_id=541714 JYG31_RS05780 WP_213418491.1 1145747..1149247(+) (addB) [Bacillus halotolerans strain MBH1]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAAGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATACGGGCGGCATGATTCGTGCTCAAGTCTTCAGCTTTTCACGACTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACGGGCGTACAGATGCTCCTGCGGAAGCTCATTGAAGAGCACAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGATAAAAGCGGTTTTACCGCACAGGTAGAACGGATGCTGACTGAGTTTAAGAGATACT
GTCTGGAACCGGAAGATATTCGACGGATGGCGGATAGCGGAACAGCTTCCGAGTATCGGGGGGAACGTATTTTATCTGAA
AAGCTTCATGATTTATCGATCCTGTACCAGCAGATGGAAAAAAGCCTTGAGGATCAGTATCTTCATTCTGAGGATTATTT
GACACTATTGGCAGAGCATATCCCGCAGGCGGAAGATTTGAAAGGGGCACATGTCTATGTAGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTAGAGCAGCTTATGCTTCAGGCAGAGCATATCACGTTTTCACTCACAGCGGACAAGCCG
TCACATGAGGGGGAGCCGCAGGAACTTGATTTGTTCAGAATGACGGGAAAAACGTATTATCGCCTCCACCAGACGGCAAA
GGAACTGAATCTGGACATCACCTATAAAGAGCTTGATGAATCAAAGCGGCACAAGCGCGCACCGGAATTAGCCCATTTAG
AAGCACAGTATGAAGCGCGTCCGGTTATTCCGTTTGCTGAGAAACAAGAGGCTTTTACCGTGATGCAGGCCGCAAACAGA
CGAGCAGAGCTGGAAGGTATTGCCCGGGAAATCCACAGCCTTGTCAGAGAGAAGGGCTACCGTTACAAGGATATGGCGAT
TCTAGCGCGTCAGCCGGAAGACTATAAGGATATGGTCAAGGAAGTATTTGCAGATTACGAGATTCCTTATTTTATTGACG
GGAAAACATCTATGCTAAACCACCCGTTAGTAGAATTTATCCGGTCAAGCCTTGACGTATTAAAGGGAAATTGGCGTTAT
GAAGCGGTGTTCCGCTGCGTGAAAACCGAACTGTTATTCCCGCTTAACCAGCCGAAGGCCAAGGTAAGAGAGCAGGTTGA
TCAGCTGGAGAATTACTGTATCGCTTACGGGATCAAAGGCGATCGCTGGACAAAGGGTGACCGTTTCCATTACAGACGTT
TTGTGTCGTTAGATGATGAATTTGCGCAGACCGATCAAGAAATCGAAATGGAAAACATGCTGAATGACACGCGCGATTGG
ATTGTTCCGCCGCTGTTCCAGCTGCAGAAACGAATGAAAAAAGCAAAAACGGTTCAAGAGAAGGCGGAGGCGCTTTACCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGGGCTGAGGAAGACGGCAGAATCATTGAAG
CGCAGCAGCACCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTGGAAATGATGGGCGAAGATGACATT
TCTCTCGATTTGTTTCAGCAAATGATGGAGACCGGTGCAGAGTCGCTGACATTCTCTCTTATCCCTCCGGCGCTTGATCA
GGTGTTTGTCGGCAATATGGATTTGTCGAGAATATACGGCACCTCATGCACCTTTATGATCGGGGCAAACGACGGCGTCC
TGCCGGCTCGCCCTGACGAAAACGGGGTGCTGTCGGATGATGATCGTGAGTGGCTGAAGACGATAGGTATTGAGCTTTCC
TCCGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGCTTTCAAGCCCGTCTGACCGCCTTTATGT
GACGTATCCGATTGCTGATGCGGAAGGGAAAACGCTTTTGCCGTCGATTGTAATCAAGCGGCTGGAAGAACTGTTTCCGC
AGCATCGTGAGCGTCTATTGACAAATGAACCGGAGCAGGTCAGCGATGAGGAACAGCTTATGTACCTTGTCAATAAAAGC
GTGGCACAATCATACACCGCTAGCCAGCTCAGATTATGGACGAGGGAATATGAAATCAGCGACGTCTGGTGGAGCGCGTA
CAATGCGCTGATGAATGAACCTGACAGGCTGGAGTCGAAAAAGCTTTTCTCTAGCCTGTTTTTCCGAAATGAAGTCAAGC
AGCTTGAACGCTCTGTATCGAGACAGCTCTATGGTGAACGCATTCAAGGGAGCGTGTCGAGGATGGAAACCTTTAACGCT
TGTCCGTTCTCCCATTTTGCGTCCCACGGGCTGCATCTGAAGGAACGTCAAGTTTTCAAGCTTGAAGCACCGGATATCGG
GCAGCTGTTTCACTCCAGCTTAAAACTGATTTCAGACCGGCTTCGTGAGCAAAAAATAGATTGGCGCGACCTAACGAAGG
AGCAGTGCGAGCTGTTTTCTTATGATGCGGTCGAGCGGCTGGCGCCGAAACTGCAAAAAGAAATTCTGCTCAGTTCAAAC
AGACATTATTACGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTATCCGGCATTTTAAGCGAGCATGCAAAAGCGAG
CGGATTCGTGCCGATCGGCCTTGAATTGGGCTTTGGCGGAAAGGGCCCGCTCCCGCCGCTTACCTTTAAGCTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGGATTGACCGCGTGGATAAAGCGGAAAGCTCTAAAGGCCTGCTCCTCCGAATTGTC
GACTATAAATCCAGCGATAAAGGCCTTGACTTGGCGGAAGTGTATTACGGACTTGCGCTGCAAATGCTGACGTATCTGGA
TTTATCGATTACACATTCGTTAGACTGGCTAGGAATGAAAGCCACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATCCAATCGAATCTTCCGCTCGGACTTGATGAAATTGAACAGGAAATTTTTAAGAAATTTAAGATGAAGGGCCTC
CTCCTTGGTGATCAGGAAGCGATTCGCCTCATGGATACAACCCTTCAAGAAGGAAGATCAAATATCATCAATGCGGGCCT
GAAGAAAGACGGCTCACTCAGATCGGATTCAGCGGCAGTCGGTGAAAAAGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAGCAAATCACTGACGGGCGCGTATCCATTGAGCCGTATAAAATGAAGAACAAGACGCCT
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGTCTGTTAAAGGCCGA
AAAGGACAAGACGGTACTTGATTGGATAAAGAAGGAGGCTGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (306-668)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	94.254	100	0.943