Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   LIO20_RS23630 Genome accession   NZ_CP085082
Coordinates   5104333..5104758 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PAO1-Holloway     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5099333..5109758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIO20_RS23615 (LIO20_23575) pilX 5099903..5100490 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  LIO20_RS23620 (LIO20_23580) pilY1 5100502..5103987 (+) 3486 WP_003115287.1 type 4a pilus biogenesis protein PilY1 -
  LIO20_RS23625 (LIO20_23585) pilY2 5103989..5104336 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  LIO20_RS23630 (LIO20_23590) comF 5104333..5104758 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  LIO20_RS23635 (LIO20_23595) ispH 5104805..5105749 (-) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  LIO20_RS23640 (LIO20_23600) fkpB 5105835..5106275 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  LIO20_RS23645 (LIO20_23605) lspA 5106268..5106777 (-) 510 WP_003112823.1 signal peptidase II -
  LIO20_RS23650 (LIO20_23610) ileS 5106770..5109601 (-) 2832 WP_003112822.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=537038 LIO20_RS23630 WP_003094721.1 5104333..5104758(+) (comF) [Pseudomonas aeruginosa strain PAO1-Holloway]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=537038 LIO20_RS23630 WP_003094721.1 5104333..5104758(+) (comF) [Pseudomonas aeruginosa strain PAO1-Holloway]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383