Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   LH369_RS21120 Genome accession   NZ_CP085060
Coordinates   4370475..4371239 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain P30_L1_05.20     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4365475..4376239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LH369_RS21100 (LH369_21100) acpT 4366429..4367016 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  LH369_RS21105 (LH369_21105) nikA 4367127..4368701 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  LH369_RS21110 (LH369_21110) nikB 4368701..4369645 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  LH369_RS21115 (LH369_21115) nikC 4369642..4370475 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  LH369_RS21120 (LH369_21120) amiE 4370475..4371239 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  LH369_RS21125 (LH369_21125) nikE 4371236..4372042 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  LH369_RS21130 (LH369_21130) nikR 4372048..4372449 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=536700 LH369_RS21120 WP_001136229.1 4370475..4371239(+) (amiE) [Escherichia coli strain P30_L1_05.20]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=536700 LH369_RS21120 WP_001136229.1 4370475..4371239(+) (amiE) [Escherichia coli strain P30_L1_05.20]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398