Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   LGS58_RS17160 Genome accession   NZ_CP084683
Coordinates   3554158..3554922 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain BL21(RP)     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3549158..3559922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGS58_RS17140 (LGS58_17140) acpT 3550112..3550699 (+) 588 WP_000285773.1 4'-phosphopantetheinyl transferase AcpT -
  LGS58_RS17145 (LGS58_17145) nikA 3550810..3552384 (+) 1575 WP_000953348.1 nickel ABC transporter substrate-binding protein -
  LGS58_RS17150 (LGS58_17150) nikB 3552384..3553328 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  LGS58_RS17155 (LGS58_17155) nikC 3553325..3554158 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  LGS58_RS17160 (LGS58_17160) amiE 3554158..3554922 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  LGS58_RS17165 (LGS58_17165) nikE 3554919..3555725 (+) 807 WP_000173665.1 nickel import ATP-binding protein NikE -
  LGS58_RS17170 (LGS58_17170) nikR 3555731..3556132 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  LGS58_RS17175 (LGS58_17175) rhsB 3556335..3559082 (+) 2748 Protein_3410 rhs element protein RhsB -
  LGS58_RS22190 - 3559077..3559139 (+) 63 Protein_3411 hypothetical protein -
  LGS58_RS17180 (LGS58_17180) pptA 3559190..3559417 (+) 228 WP_001120141.1 tautomerase PptA -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=535145 LGS58_RS17160 WP_001136236.1 3554158..3554922(+) (amiE) [Escherichia coli strain BL21(RP)]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=535145 LGS58_RS17160 WP_001136236.1 3554158..3554922(+) (amiE) [Escherichia coli strain BL21(RP)]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398