Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   LFW28_RS19265 Genome accession   NZ_CP084529
Coordinates   4001257..4002021 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain Origami2-DE3     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3996257..4007021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFW28_RS19245 (LFW28_19245) acpT 3997211..3997798 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  LFW28_RS19250 (LFW28_19250) nikA 3997909..3999483 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  LFW28_RS19255 (LFW28_19255) nikB 3999483..4000427 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  LFW28_RS19260 (LFW28_19260) nikC 4000424..4001257 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  LFW28_RS19265 (LFW28_19265) amiE 4001257..4002021 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  LFW28_RS19270 (LFW28_19270) nikE 4002018..4002824 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  LFW28_RS19275 (LFW28_19275) nikR 4002830..4003231 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  LFW28_RS19280 (LFW28_19280) - 4003434..4003640 (+) 207 Protein_3793 hypothetical protein -
  LFW28_RS19285 (LFW28_19285) - 4003695..4005686 (+) 1992 Protein_3794 catecholate siderophore receptor Fiu -
  LFW28_RS19290 (LFW28_19290) ybiX 4005728..4006405 (+) 678 WP_000990177.1 PKHD-type hydroxylase YbiX -
  LFW28_RS19295 (LFW28_19295) ybiI 4006479..4006745 (+) 267 WP_000146343.1 C4-type zinc finger protein YbiI -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=535073 LFW28_RS19265 WP_001136229.1 4001257..4002021(+) (amiE) [Escherichia coli strain Origami2-DE3]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=535073 LFW28_RS19265 WP_001136229.1 4001257..4002021(+) (amiE) [Escherichia coli strain Origami2-DE3]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398