Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   LDB15_RS05030 Genome accession   NZ_CP084207
Coordinates   1075093..1075857 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain EC2648-R1-1     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1070093..1080857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDB15_RS05020 (LDB15_05020) nikR 1073883..1074284 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  LDB15_RS05025 (LDB15_05025) nikE 1074290..1075096 (-) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  LDB15_RS05030 (LDB15_05030) amiE 1075093..1075857 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  LDB15_RS05035 (LDB15_05035) nikC 1075857..1076690 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  LDB15_RS05040 (LDB15_05040) nikB 1076687..1077631 (-) 945 WP_072647353.1 nickel ABC transporter permease subunit NikB -
  LDB15_RS05045 (LDB15_05045) nikA 1077631..1079205 (-) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  LDB15_RS05050 (LDB15_05050) acpT 1079316..1079903 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=534604 LDB15_RS05030 WP_001136229.1 1075093..1075857(-) (amiE) [Escherichia coli strain EC2648-R1-1]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=534604 LDB15_RS05030 WP_001136229.1 1075093..1075857(-) (amiE) [Escherichia coli strain EC2648-R1-1]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398