Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ICR95_RS15400 Genome accession   NZ_CP069397
Coordinates   2952572..2954914 (-) Length   780 a.a.
NCBI ID   WP_028411690.1    Uniprot ID   A0AAX6BQR2
Organism   Priestia megaterium strain CDC 2008724129     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2947572..2959914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICR95_RS15375 (ICR95_15375) - 2947965..2948141 (-) 177 WP_013059305.1 hypothetical protein -
  ICR95_RS15380 (ICR95_15380) - 2948308..2949192 (+) 885 WP_223546263.1 N-acetylmuramoyl-L-alanine amidase -
  ICR95_RS15385 (ICR95_15385) dtd 2949183..2949644 (-) 462 WP_028411689.1 D-aminoacyl-tRNA deacylase -
  ICR95_RS15390 (ICR95_15390) - 2949660..2951858 (-) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  ICR95_RS15395 (ICR95_15395) - 2952044..2952556 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  ICR95_RS15400 (ICR95_15400) recJ 2952572..2954914 (-) 2343 WP_028411690.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ICR95_RS15405 (ICR95_15405) secDF 2955055..2957292 (-) 2238 WP_028411691.1 protein translocase subunit SecDF -
  ICR95_RS15410 (ICR95_15410) - 2957430..2957723 (-) 294 WP_028411692.1 post-transcriptional regulator -
  ICR95_RS15415 (ICR95_15415) spoVB 2957788..2959398 (+) 1611 WP_028411693.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87937.54 Da        Isoelectric Point: 4.7742

>NTDB_id=534452 ICR95_RS15400 WP_028411690.1 2952572..2954914(-) (recJ) [Priestia megaterium strain CDC 2008724129]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADTDFYIPNRFTEGYGPNKAAFDKINAGGYQLVITVDTGISAVEETAYAREL
DFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEITEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVESMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSVDSENRIAKGSARSIQGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSEDVEELRRRLNEQASNVLTQQDFIPITSVDTVCKTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFEQVFTDQMKVVAFHDSTIDKYKSMLPMEHVVLIQNEEQAKACMFAGEQIVLLDLPPSQ
KWLEALFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSTKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=534452 ICR95_RS15400 WP_028411690.1 2952572..2954914(-) (recJ) [Priestia megaterium strain CDC 2008724129]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGTACTTCAGTTATGTTATCTGCTTTACAGCAGCT
CGGCGCTGATACTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
ACGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACGGCGTATGCACGTGAATTA
GATTTTGATTTAATTATTACTGATCACCACGAGCCGGGTCCGGTTTTACCCGATGCCTATGCAATTATTCATCCAAAGCT
GCCGGGCAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTTGCTTTTAAACTTGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACGAAGCGTACTGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
CGAAGAAATTACTGAAGATACAATTGGATTTGCCATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCATTTGCTTATGACAACAAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACGGAAGAAGCCGTTGAATTTGTTGAAAGTATGTATCCTCCGGATGAAAATAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTTGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTATTAAGTGTTGACTCTGAAAACCGTATTGCTAAAGGTTCAGCAAGAAGTATCCAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACGTTAAACAGCGA
GGATGTAGAAGAACTGCGCAGACGCCTTAATGAACAAGCAAGCAACGTGTTAACTCAACAGGACTTTATTCCAATTACAT
CAGTTGATACAGTATGCAAAACAGAGGAAATTACCCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAATCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCGAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAGGTATCATTAGTAGGTGAACTATCCATTAATGAGTGGAACAATATGCGCAAACCGCAGCTGATGGTAGAA
GATATCTCTGTAGATGAGTGGCAGATGTTTGATTATCGCAGCGCGGTTGATGTCAAACGTTTCTTTGAACAGGTATTCAC
AGATCAAATGAAAGTTGTTGCTTTTCACGATTCAACAATTGATAAATACAAGTCGATGCTGCCGATGGAACATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCATGCATGTTTGCAGGAGAACAGATCGTTTTATTAGATTTGCCTCCGTCACAG
AAGTGGCTTGAAGCTTTGTTTGTCCAAGGTATTCCTTCTAGGATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCACTTTAAATGGTTCTACGGCTTTTTAGCGAAAAAGGGTCCTTTTGATTTAAAACGCT
ACAAAGAAGATTTAGCAATTCATAAGGGCTGGTCGAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTACGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(305-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.385

100

0.554