Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   LCF29_RS01675 Genome accession   NZ_CP084096
Coordinates   346076..346840 (-) Length   254 a.a.
NCBI ID   WP_001136218.1    Uniprot ID   -
Organism   Escherichia coli O78 strain 3     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 341076..351840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCF29_RS01645 (LCF29_01645) - 341710..343068 (-) 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
  LCF29_RS01650 (LCF29_01650) - 343145..343426 (-) 282 WP_000084022.1 PTS sugar transporter subunit IIB -
  LCF29_RS01655 (LCF29_01655) - 343423..343896 (-) 474 WP_001161550.1 PTS sugar transporter subunit IIA -
  LCF29_RS01660 (LCF29_01660) - 343921..344667 (-) 747 WP_001603991.1 GntR family transcriptional regulator -
  LCF29_RS01665 (LCF29_01665) nikR 344866..345267 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  LCF29_RS01670 (LCF29_01670) nikE 345273..346079 (-) 807 WP_000173708.1 nickel import ATP-binding protein NikE -
  LCF29_RS01675 (LCF29_01675) amiE 346076..346840 (-) 765 WP_001136218.1 nickel import ATP-binding protein NikD Regulator
  LCF29_RS01680 (LCF29_01680) nikC 346840..347673 (-) 834 WP_001008803.1 nickel ABC transporter permease subunit NikC -
  LCF29_RS01685 (LCF29_01685) nikB 347670..348614 (-) 945 WP_000947059.1 nickel ABC transporter permease subunit NikB -
  LCF29_RS01690 (LCF29_01690) nikA 348614..350188 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  LCF29_RS01695 (LCF29_01695) acpT 350299..350886 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26782.38 Da        Isoelectric Point: 6.6882

>NTDB_id=534159 LCF29_RS01675 WP_001136218.1 346076..346840(-) (amiE) [Escherichia coli O78 strain 3]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=534159 LCF29_RS01675 WP_001136218.1 346076..346840(-) (amiE) [Escherichia coli O78 strain 3]
ATGCCGCAACAGATTGAACTGCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGGCGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCACCGGGGATGCTGCTGGTCACCCATGATATGGGCGTAGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCGCACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGCGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398