Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   LCR13_RS12815 Genome accession   NZ_CP084055
Coordinates   2587290..2588054 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain X14-3     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2582290..2593054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCR13_RS12795 (LCR13_12810) acpT 2583244..2583831 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  LCR13_RS12800 (LCR13_12815) nikA 2583942..2585516 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  LCR13_RS12805 (LCR13_12820) nikB 2585516..2586460 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  LCR13_RS12810 (LCR13_12825) nikC 2586457..2587290 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  LCR13_RS12815 (LCR13_12830) amiE 2587290..2588054 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  LCR13_RS12820 (LCR13_12835) nikE 2588051..2588857 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  LCR13_RS12825 (LCR13_12840) nikR 2588863..2589264 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=533935 LCR13_RS12815 WP_001136229.1 2587290..2588054(+) (amiE) [Escherichia coli strain X14-3]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=533935 LCR13_RS12815 WP_001136229.1 2587290..2588054(+) (amiE) [Escherichia coli strain X14-3]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398