Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   LA372_RS01235 Genome accession   NZ_CP083585
Coordinates   264838..265602 (-) Length   254 a.a.
NCBI ID   WP_047623513.1    Uniprot ID   -
Organism   Escherichia coli strain ECNB21-M121     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 259838..270602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA372_RS01210 (LA372_01210) yhhJ 261680..262804 (+) 1125 WP_001314210.1 ABC transporter permease -
  LA372_RS01215 (LA372_01215) - 262877..263152 (+) 276 WP_047623515.1 type II toxin-antitoxin system HicA family toxin -
  LA372_RS01220 (LA372_01220) - 263149..263508 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  LA372_RS01225 (LA372_01225) nikR 263628..264029 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  LA372_RS01230 (LA372_01230) nikE 264035..264841 (-) 807 WP_047623514.1 nickel import ATP-binding protein NikE -
  LA372_RS01235 (LA372_01235) amiE 264838..265602 (-) 765 WP_047623513.1 nickel import ATP-binding protein NikD Regulator
  LA372_RS01240 (LA372_01240) nikC 265602..266435 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  LA372_RS01245 (LA372_01245) nikB 266432..267376 (-) 945 WP_047623512.1 nickel ABC transporter permease subunit NikB -
  LA372_RS01250 (LA372_01250) nikA 267376..268950 (-) 1575 WP_000953368.1 nickel ABC transporter substrate-binding protein -
  LA372_RS01255 (LA372_01255) acpT 269062..269649 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26802.36 Da        Isoelectric Point: 6.0038

>NTDB_id=532710 LA372_RS01235 WP_047623513.1 264838..265602(-) (amiE) [Escherichia coli strain ECNB21-M121]
MPQQIELRDIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAAALGILPAGVRQTAGEILADGKQVSPCALRGIK
IATIMQNPRSAFNPLRTMQAHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=532710 LA372_RS01235 WP_047623513.1 264838..265602(-) (amiE) [Escherichia coli strain ECNB21-M121]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGGCGCTGGGAATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGAAAACAGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACGCACCATGCAGGCCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCACCGGGGATGCTGCTGGTCACCCATGATATGGGCGTAGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402