Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHHHKEFG_RS11980 Genome accession   NZ_CP069125
Coordinates   2357865..2360189 (-) Length   774 a.a.
NCBI ID   WP_066030600.1    Uniprot ID   -
Organism   Bacillus pumilus strain D5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2352865..2365189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHHHKEFG_RS11965 (MHHHKEFG_02443) dtd 2354514..2354957 (-) 444 WP_060596866.1 D-aminoacyl-tRNA deacylase -
  MHHHKEFG_RS11970 (MHHHKEFG_02444) - 2354976..2357186 (-) 2211 WP_060596867.1 RelA/SpoT family protein -
  MHHHKEFG_RS11975 (MHHHKEFG_02445) - 2357329..2357841 (-) 513 WP_034661543.1 adenine phosphoribosyltransferase -
  MHHHKEFG_RS11980 (MHHHKEFG_02446) recJ 2357865..2360189 (-) 2325 WP_066030600.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHHHKEFG_RS11985 (MHHHKEFG_02447) - 2360271..2360624 (-) 354 WP_060596869.1 lipopolysaccharide assembly LapA domain-containing protein -
  MHHHKEFG_RS11990 (MHHHKEFG_02448) - 2360726..2361214 (-) 489 WP_066030599.1 cation:proton antiporter regulatory subunit -
  MHHHKEFG_RS11995 (MHHHKEFG_02449) secDF 2361402..2363630 (-) 2229 WP_060596871.1 protein translocase subunit SecDF -
  MHHHKEFG_RS12000 (MHHHKEFG_02450) - 2363672..2363968 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87397.06 Da        Isoelectric Point: 4.9283

>NTDB_id=532396 MHHHKEFG_RS11980 WP_066030600.1 2357865..2360189(-) (recJ) [Bacillus pumilus strain D5]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARVAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPAQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELACFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDRFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMQN
PKPFIMIEDARLEEIRTIGANQNHIKMSLKDEDKLLDCVGFHQGQLKEEIVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKQEVNIPIYHVHAENVSTFDVEGKYIVFADVPADEHLLRRLLE
NQKPARVYTVFQRKEDHFMSSFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKETIIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=532396 MHHHKEFG_RS11980 WP_066030600.1 2357865..2360189(-) (recJ) [Bacillus pumilus strain D5]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGGATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGCG
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTTCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACGGTCGATACAGGCATTGCAGCTGTAAATGAAGCACGTGTTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCGATTGTCCATCCTAAGCA
GCCGGGCTGTGAGTATCCTTTTAAAGAACTTGCCGGTGTAGGCGTAGCGTTTAAAGTGGCACATGCACTTCTTGGGAAAC
TGCCAGCTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
GCCAAAAAAGGGCTTGTGCAGCTTCGACAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCTACTCT
TGAAGAAGCAAATGAAGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGGCGAATTGAGCAGGCAGATC
CAGCTGTTCATCTTCTCATGACAGAGGATAAGGATGAAGCAGAAGAGCTCGCTTGTTTTGTACAAGAACTCAATAAAGAA
CGTCAAAAAATTGTGAGTACAATCACAGAAGAAGCCATTCAAATGGTTGAAGAGACAGGTGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATAGATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCGCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGTGACATTCTGCCGCATTTCGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGAGCGGATGATGT
TCCTTTACTAAGAGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAAGAAGATTTTATTCCGATTCAGCGGGTCG
ATGCCGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGCGAGGCTTGAAGAGATCCGAACGATTGGGGCAAATCAGAATCATATTAAAAT
GTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCATCAAGGCCAATTAAAAGAAGAAATTGTCACAGGTA
GCCGCATTTCCGTCATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCTCAGCTTATGCTAAAAGATGCC
CGAGTAGATGAATGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACAATCTCCACGCTTGATCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAACAGGAAGTCAATATCCCAATTTATCATGTCCATGCAGAAAATGTTT
CAACGTTCGATGTAGAGGGCAAGTATATCGTCTTTGCGGATGTTCCTGCAGATGAGCATTTGCTAAGGCGACTTTTAGAA
AACCAAAAGCCAGCTAGAGTTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCTTCTTTCCCTAGCCGTGATCA
ATTCAAATGGTTTTATGGATTCTTGTTAAAACGAGGAAGTTTCCCCCTCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGAAACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

DHH family (DHH)

(83-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.825

100

0.681