Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   JNE32_RS13765 Genome accession   NZ_CP068982
Coordinates   2603079..2604116 (-) Length   345 a.a.
NCBI ID   WP_029726718.1    Uniprot ID   -
Organism   Bacillus subtilis strain pb2441     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2598079..2609116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNE32_RS13715 (JNE32_13650) tasA 2598177..2598962 (-) 786 WP_014664586.1 biofilm matrix protein TasA -
  JNE32_RS13720 (JNE32_13655) sipW 2599026..2599598 (-) 573 WP_072692741.1 signal peptidase I SipW -
  JNE32_RS13725 (JNE32_13660) tapA 2599582..2600343 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  JNE32_RS13730 (JNE32_13665) yqzG 2600614..2600940 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  JNE32_RS13735 (JNE32_13670) spoIITA 2600982..2601161 (-) 180 WP_029726723.1 YqzE family protein -
  JNE32_RS13740 (JNE32_13675) comGG 2601233..2601607 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  JNE32_RS13745 (JNE32_13680) comGF 2601608..2601991 (-) 384 WP_029726721.1 ComG operon protein ComGF Machinery gene
  JNE32_RS13750 (JNE32_13685) comGE 2602017..2602364 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  JNE32_RS13755 (JNE32_13690) comGD 2602348..2602779 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  JNE32_RS13760 (JNE32_13695) comGC 2602769..2603065 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  JNE32_RS13765 (JNE32_13700) comGB 2603079..2604116 (-) 1038 WP_029726718.1 comG operon protein ComGB Machinery gene
  JNE32_RS13770 (JNE32_13705) comGA 2604103..2605172 (-) 1070 Protein_2674 competence protein ComGA -
  JNE32_RS13775 (JNE32_13710) - 2605385..2605582 (-) 198 WP_029726717.1 hypothetical protein -
  JNE32_RS13780 (JNE32_13715) corA 2605584..2606537 (-) 954 WP_004399136.1 magnesium transporter CorA -
  JNE32_RS13785 (JNE32_13720) yqhB 2606680..2608008 (+) 1329 WP_029726716.1 hemolysin family protein -
  JNE32_RS13790 (JNE32_13725) rsbRD 2608061..2608897 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39702.10 Da        Isoelectric Point: 9.6257

>NTDB_id=531741 JNE32_RS13765 WP_029726718.1 2603079..2604116(-) (comGB) [Bacillus subtilis strain pb2441]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDILFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=531741 JNE32_RS13765 WP_029726718.1 2603079..2604116(-) (comGB) [Bacillus subtilis strain pb2441]
ATGAAAAAGATTAGAAAAGTCTGGTCGTTAAAGGATCAAGCGAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATACTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTCAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.381

93.623

0.93