Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   K6U01_RS08010 Genome accession   NZ_CP083274
Coordinates   1685289..1686053 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain Rosetta2-DE3     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1680289..1691053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6U01_RS07990 (K6U01_07970) acpT 1681243..1681830 (+) 588 WP_000285773.1 4'-phosphopantetheinyl transferase AcpT -
  K6U01_RS07995 (K6U01_07975) nikA 1681941..1683515 (+) 1575 WP_000953348.1 nickel ABC transporter substrate-binding protein -
  K6U01_RS08000 (K6U01_07980) nikB 1683515..1684459 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  K6U01_RS08005 (K6U01_07985) nikC 1684456..1685289 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  K6U01_RS08010 (K6U01_07990) amiE 1685289..1686053 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  K6U01_RS08015 (K6U01_07995) nikE 1686050..1686856 (+) 807 WP_000173665.1 nickel import ATP-binding protein NikE -
  K6U01_RS08020 (K6U01_08000) nikR 1686862..1687263 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  K6U01_RS08025 (K6U01_08005) rhsB 1687466..1690216 (+) 2751 Protein_1592 rhs element protein RhsB -
  K6U01_RS08030 (K6U01_08010) yjdN 1690570..1691013 (+) 444 WP_001131339.1 VOC family metalloprotein YjdN -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=531641 K6U01_RS08010 WP_001136236.1 1685289..1686053(+) (amiE) [Escherichia coli strain Rosetta2-DE3]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=531641 K6U01_RS08010 WP_001136236.1 1685289..1686053(+) (amiE) [Escherichia coli strain Rosetta2-DE3]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398