Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   JM956_RS05945 Genome accession   NZ_CP068719
Coordinates   1144365..1147880 (+) Length   1171 a.a.
NCBI ID   WP_202822473.1    Uniprot ID   -
Organism   Bacillus cereus strain 21155     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1139365..1152880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JM956_RS05905 - 1139525..1140019 (-) 495 WP_000182384.1 DUF6584 family protein -
  JM956_RS05910 cspA 1140303..1140506 (+) 204 WP_000301523.1 RNA chaperone/antiterminator CspA -
  JM956_RS05915 - 1140707..1140910 (-) 204 WP_000356609.1 hypothetical protein -
  JM956_RS05920 - 1141261..1141446 (-) 186 WP_000391367.1 hypothetical protein -
  JM956_RS05925 - 1141731..1142312 (+) 582 WP_131250263.1 competence protein ComK -
  JM956_RS05930 - 1142383..1142682 (-) 300 WP_000655757.1 hypothetical protein -
  JM956_RS05935 - 1143035..1143628 (+) 594 WP_088022721.1 TVP38/TMEM64 family protein -
  JM956_RS05940 lepB 1143685..1144248 (+) 564 WP_000751900.1 signal peptidase I -
  JM956_RS05945 addB 1144365..1147880 (+) 3516 WP_202822473.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  JM956_RS05950 addA 1147877..1151602 (+) 3726 WP_202822474.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  JM956_RS05955 - 1151615..1151902 (+) 288 WP_000179952.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  JM956_RS05960 gerPF 1152040..1152255 (-) 216 WP_001141566.1 spore germination protein GerPF -
  JM956_RS05965 - 1152298..1152684 (-) 387 WP_000902323.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134167.32 Da        Isoelectric Point: 5.1418

>NTDB_id=530681 JM956_RS05945 WP_202822473.1 1144365..1147880(+) (addB) [Bacillus cereus strain 21155]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLSSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTVTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNDEASVTISTAANL
RAEVEGVAREIRKLVADEKYRYRDIAVLLRNGGSYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGDEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAKKLSTAVSRDLYGDIIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRSLKDMKDNEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=530681 JM956_RS05945 WP_202822473.1 1144365..1147880(+) (addB) [Bacillus cereus strain 21155]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCGAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTACTACAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGGTT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTGAGCAGTATGATTGCAGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAGCAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CTTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGATGGATTTCATTCAT
TCTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGGTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAAAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCCCATTATGAAGCGCGTCCGAATGAAAAATTTAACGATGAAGCGAGTGTAACGATTAGTACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGTGTTGCTCGTGAAATTCGTAAACTTGTTGCGGATGAAAAATATCGTTACCGAGATATTGCAGT
ACTTCTTCGTAATGGGGGAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTACAATATCCCGCACTTCATCGATG
AAAAGCGCCCGATGTCACACCATCCATTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTTCAGAGGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATCACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGCTACGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTATTTTTAGAAGAGCTGGATGTTCCAAAGAAACTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAGGTAATGAGCCTTCTTGATACGTTTGTAGAGATGCTTGGTGATGAGAAA
ATGTCTCTTTCTATGTTTACGGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCTAATATTCCGCCTTCATTAGA
CCAAGTATTAATTGCTAATATTGATCACTCCAGGTTATCTGACGTGAAAGCGACGTTTATTATAGGTGTAAATGAGGGGG
TTATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCGGCAGGAATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAGTTATA
CATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCATTTATTACGAATGACGTAAATGATTTATCACGTTCAGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATACAACTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTCTTATCAAGTTTATTCTATCGAA
ATCGTGCGAAGAAACTAAGTACAGCAGTAAGTAGAGATTTATATGGAGATATAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGTGCATACGCTCATTTTGCGCAGCACGGTTTATCGCTTAGAGAGCGTGATATTTTCAAGCTTGATGC
GCCAGATATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGTTATTGCGTGAAAACCGTACTTGGGCGG
ATTTATCCATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATCGCACCGTTATTACAACGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCGAGCGGATTCGTACCAGTTGATTTAGAAGTGCCGTTCGGTATGGGCGGTACAGGATCACTTCCGCCGATGG
AATTCTCACTACCAAATGGTGTGAAGATGGAAGTAGTCGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCACAGACCTGGATGAAAAAAGGTCACACAGCATCACCAGCTGGTG
TATTGTATTTTCATATTCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCGGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAGTCACTTGAAGATAA
TCAGTTCCGATCGTTAAAAGATATGAAAGATAACGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.317

100

0.494