Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   K8B71_RS29055 Genome accession   NZ_CP082823
Coordinates   5996250..5996675 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5991250..6001675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B71_RS29040 (K8B71_29040) pilX 5991820..5992407 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  K8B71_RS29045 (K8B71_29045) pilY1 5992419..5995904 (+) 3486 WP_003134937.1 type 4a pilus biogenesis protein PilY1 -
  K8B71_RS29050 (K8B71_29050) pilY2 5995906..5996253 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  K8B71_RS29055 (K8B71_29055) comF 5996250..5996675 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  K8B71_RS29060 (K8B71_29060) ispH 5996722..5997666 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  K8B71_RS29065 (K8B71_29065) fkpB 5997752..5998192 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  K8B71_RS29070 (K8B71_29070) lspA 5998185..5998694 (-) 510 WP_003094728.1 signal peptidase II -
  K8B71_RS29075 (K8B71_29075) ileS 5998687..6001518 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=529951 K8B71_RS29055 WP_003094721.1 5996250..5996675(+) (comF) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=529951 K8B71_RS29055 WP_003094721.1 5996250..5996675(+) (comF) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCAGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTAGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383