Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   K8B69_RS25410 Genome accession   NZ_CP082822
Coordinates   5339477..5339902 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5334477..5344902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B69_RS25395 (K8B69_25395) pilX 5335041..5335628 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  K8B69_RS25400 (K8B69_25400) pilY1 5335640..5339131 (+) 3492 WP_010793785.1 type 4a pilus biogenesis protein PilY1 -
  K8B69_RS25405 (K8B69_25405) pilY2 5339133..5339480 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  K8B69_RS25410 (K8B69_25410) comF 5339477..5339902 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  K8B69_RS25415 (K8B69_25415) ispH 5339949..5340893 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  K8B69_RS25420 (K8B69_25420) fkpB 5340979..5341419 (-) 441 WP_003161775.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  K8B69_RS25425 (K8B69_25425) lspA 5341412..5341921 (-) 510 WP_003102615.1 signal peptidase II -
  K8B69_RS25430 (K8B69_25430) ileS 5341914..5344745 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=529840 K8B69_RS25410 WP_003094721.1 5339477..5339902(+) (comF) [Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=529840 K8B69_RS25410 WP_003094721.1 5339477..5339902(+) (comF) [Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383