Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   K8B91_RS23070 Genome accession   NZ_CP082777
Coordinates   4788480..4789244 (+) Length   254 a.a.
NCBI ID   WP_001136233.1    Uniprot ID   Q8FCN0
Organism   Escherichia coli O25:H1 strain CU39ES-1     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4783480..4794244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B91_RS23050 (K8B91_22930) acpT 4784434..4785021 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  K8B91_RS23055 (K8B91_22935) nikA 4785132..4786706 (+) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  K8B91_RS23060 (K8B91_22940) nikB 4786706..4787650 (+) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  K8B91_RS23065 (K8B91_22945) nikC 4787647..4788480 (+) 834 WP_001008953.1 nickel ABC transporter permease subunit NikC -
  K8B91_RS23070 (K8B91_22950) amiE 4788480..4789244 (+) 765 WP_001136233.1 nickel import ATP-binding protein NikD Regulator
  K8B91_RS23075 (K8B91_22955) nikE 4789241..4790047 (+) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  K8B91_RS23080 (K8B91_22960) nikR 4790053..4790454 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  K8B91_RS23085 (K8B91_22965) - 4790653..4791399 (+) 747 WP_001304937.1 GntR family transcriptional regulator -
  K8B91_RS23090 (K8B91_22970) - 4791424..4791897 (+) 474 WP_001443177.1 PTS sugar transporter subunit IIA -
  K8B91_RS23095 (K8B91_22975) - 4791894..4792175 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  K8B91_RS23100 (K8B91_22980) - 4792252..4793610 (+) 1359 WP_001304938.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26840.46 Da        Isoelectric Point: 6.6882

>NTDB_id=529077 K8B91_RS23070 WP_001136233.1 4788480..4789244(+) (amiE) [Escherichia coli O25:H1 strain CU39ES-1]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNVPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=529077 K8B91_RS23070 WP_001136233.1 4788480..4789244(+) (amiE) [Escherichia coli O25:H1 strain CU39ES-1]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTTGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGTCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FCN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398