Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   K8B89_RS23375 Genome accession   NZ_CP082775
Coordinates   4711856..4712620 (+) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli O25:H4 strain CU568-3     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4706856..4717620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B89_RS23355 (K8B89_23195) acpT 4707810..4708397 (+) 588 WP_000285760.1 4'-phosphopantetheinyl transferase AcpT -
  K8B89_RS23360 (K8B89_23200) nikA 4708508..4710082 (+) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  K8B89_RS23365 (K8B89_23205) nikB 4710082..4711026 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  K8B89_RS23370 (K8B89_23210) nikC 4711023..4711856 (+) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  K8B89_RS23375 (K8B89_23215) amiE 4711856..4712620 (+) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  K8B89_RS23380 (K8B89_23220) nikE 4712617..4713423 (+) 807 WP_000173684.1 nickel import ATP-binding protein NikE -
  K8B89_RS23385 (K8B89_23225) nikR 4713429..4713830 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  K8B89_RS23390 (K8B89_23230) - 4714029..4714775 (+) 747 WP_001296489.1 UTRA domain-containing protein -
  K8B89_RS23395 (K8B89_23235) - 4714800..4715273 (+) 474 WP_001161648.1 PTS sugar transporter subunit IIA -
  K8B89_RS23400 (K8B89_23240) - 4715270..4715551 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  K8B89_RS23405 (K8B89_23245) - 4715628..4716986 (+) 1359 WP_001525857.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=528904 K8B89_RS23375 WP_001136232.1 4711856..4712620(+) (amiE) [Escherichia coli O25:H4 strain CU568-3]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=528904 K8B89_RS23375 WP_001136232.1 4711856..4712620(+) (amiE) [Escherichia coli O25:H4 strain CU568-3]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAAGCACGCATCCTCGATCTACTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTAGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCGCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398