Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   K7X31_RS01265 Genome accession   NZ_CP082357
Coordinates   292302..293066 (-) Length   254 a.a.
NCBI ID   WP_217800989.1    Uniprot ID   -
Organism   Escherichia coli strain VCSW7     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 287302..298066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7X31_RS01240 (K7X31_01235) yhhJ 289145..290269 (+) 1125 WP_001216257.1 ABC transporter permease -
  K7X31_RS01245 (K7X31_01240) - 290342..290617 (+) 276 WP_223024845.1 type II toxin-antitoxin system HicA family toxin -
  K7X31_RS01250 (K7X31_01245) - 290614..290973 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  K7X31_RS01255 (K7X31_01250) nikR 291093..291494 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  K7X31_RS01260 (K7X31_01255) nikE 291499..292305 (-) 807 WP_000173690.1 nickel import ATP-binding protein NikE -
  K7X31_RS01265 (K7X31_01260) amiE 292302..293066 (-) 765 WP_217800989.1 nickel import ATP-binding protein NikD Regulator
  K7X31_RS01270 (K7X31_01265) nikC 293066..293899 (-) 834 WP_001008967.1 nickel ABC transporter permease subunit NikC -
  K7X31_RS01275 (K7X31_01270) nikB 293896..294840 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  K7X31_RS01280 (K7X31_01275) nikA 294840..296414 (-) 1575 WP_223024846.1 nickel ABC transporter substrate-binding protein -
  K7X31_RS01285 (K7X31_01280) acpT 296525..297112 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.45 Da        Isoelectric Point: 6.9016

>NTDB_id=528405 K7X31_RS01265 WP_217800989.1 292302..293066(-) (amiE) [Escherichia coli strain VCSW7]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAALEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=528405 K7X31_RS01265 WP_217800989.1 292302..293066(-) (amiE) [Escherichia coli strain VCSW7]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGTGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTCC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAG
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCTTTAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACTACCGACCTTGATGTGGTAGCGCAGGCGCGCATCCTCGATCTGCTGGAGAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTAGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCGCACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398