Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   K6125_RS18585 Genome accession   NZ_CP082077
Coordinates   3829089..3829853 (-) Length   254 a.a.
NCBI ID   WP_001136233.1    Uniprot ID   Q8FCN0
Organism   Escherichia coli strain MS20657     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3824089..3834853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6125_RS18570 (K6125_18455) yhhJ 3826746..3827870 (+) 1125 WP_001296492.1 ABC transporter permease -
  K6125_RS18575 (K6125_18460) nikR 3827879..3828280 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  K6125_RS18580 (K6125_18465) nikE 3828286..3829092 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  K6125_RS18585 (K6125_18470) amiE 3829089..3829853 (-) 765 WP_001136233.1 nickel import ATP-binding protein NikD Regulator
  K6125_RS18590 (K6125_18475) nikC 3829853..3830686 (-) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  K6125_RS18595 (K6125_18480) nikB 3830683..3831627 (-) 945 WP_001553570.1 nickel ABC transporter permease subunit NikB -
  K6125_RS18600 (K6125_18485) nikA 3831627..3833201 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  K6125_RS18605 (K6125_18490) acpT 3833312..3833899 (-) 588 WP_000285790.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26840.46 Da        Isoelectric Point: 6.6882

>NTDB_id=525481 K6125_RS18585 WP_001136233.1 3829089..3829853(-) (amiE) [Escherichia coli strain MS20657]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNVPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=525481 K6125_RS18585 WP_001136233.1 3829089..3829853(-) (amiE) [Escherichia coli strain MS20657]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGACGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGTCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FCN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398