Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BTF1_RS20235 Genome accession   NC_018508
Coordinates   3983757..3986096 (-) Length   779 a.a.
NCBI ID   WP_000941232.1    Uniprot ID   A0AB35PC10
Organism   Bacillus thuringiensis HD-789     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3978757..3991096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTF1_RS35595 (BTF1_20630) - 3979639..3979815 (-) 177 WP_001242605.1 hypothetical protein -
  BTF1_RS20215 (BTF1_20635) - 3979898..3980230 (-) 333 WP_000938751.1 YrdB family protein -
  BTF1_RS20220 (BTF1_20640) - 3980346..3980786 (-) 441 WP_001266969.1 D-tyrosyl-tRNA(Tyr) deacylase -
  BTF1_RS20225 (BTF1_20645) relA 3980803..3982986 (-) 2184 WP_001262795.1 GTP diphosphokinase -
  BTF1_RS20230 (BTF1_20650) - 3983197..3983709 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  BTF1_RS20235 (BTF1_20655) recJ 3983757..3986096 (-) 2340 WP_000941232.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BTF1_RS20240 (BTF1_20660) - 3986198..3987091 (-) 894 WP_000409738.1 cation diffusion facilitator family transporter -
  BTF1_RS20245 (BTF1_20665) secDF 3987248..3989512 (-) 2265 WP_001119077.1 protein translocase subunit SecDF -
  BTF1_RS20250 (BTF1_20670) - 3989782..3990066 (-) 285 WP_000884549.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88178.70 Da        Isoelectric Point: 4.8444

>NTDB_id=52493 BTF1_RS20235 WP_000941232.1 3983757..3986096(-) (recJ) [Bacillus thuringiensis HD-789]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGLYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLEDYKEKLMHASEVTHLDDQYIVLLDLPNGTDELRE
LFKVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=52493 BTF1_RS20235 WP_000941232.1 3983757..3986096(-) (recJ) [Bacillus thuringiensis HD-789]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAGCGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCATTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGATTACGATGCGGACGGAGTAAGTAGTACGACGGTTTTATATCTTGCTCTTCAAGAATT
AGGTGCAGAAGTAGAATTTTACATTCCAAATCGTTTTACAGAAGGGTATGGACCGAACGAAGAAGCGTTTCGTTGGGCGC
ATAGCGCAGGGTTCTCACTGATTATTACTGTTGACACTGGCATCGCAGCAGTACATGAAGCGAATGTAGCAAAGGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCACTTGCGATTATACATCCGAAATT
AGAAGGTGGCCTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCACTATTAGGCCGTG
TACCAGAACATTTATTAGAGATTGCAGTAATCGGTACAGTAGCCGATTTAGTTTCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGCGGTTTAAAACATATGCGTATGACGAAAAATATTGGTTTGAAGGCATTGTTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATTGCACCGCGTATTAATGCGGTCGGGCGTTTAGAAGATGCAACGC
CGGCTGTACACCTTTTATTATCAGATGATCCTGAGGAAGCGAAAGAGTTGGCCGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACCGAAGAGGCTATCGCTGAAGTTGAAAATAACTTTCCGCCAGAAGAAAACAAAGT
ATTAGTACTTGCAAAAGAAGGTTGGAATCCAGGTGTAATTGGGATTGTCGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACTATTGTATTATGCATTGATCCTATAAAAGAAACAGCAAAAGGCTCAGCGCGTAGTATTGCAGGATTTGATTTGTTT
GCAAATTTATCAGATTGTAGAGAGTTATTGCCACACTTCGGAGGGCATCCGATGGCAGCTGGGATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGTCGCTTAAATGAACAGGCAGATGCAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGTAAAGTGGAGGATGTAACGCTAGCGGCAATTGAAGATATGCAGAAGTTAGCACCATTTGGTGTT
GGAAATCCGAAACCACGTATTGCAGTGAAAGATGCAGAGTTAGAAAGTATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTCGGTTTCGGTGCATATGCGAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCATCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAACTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGGACTATAAAGAAAAGCTTATGC
ATGCGTCAGAAGTAACGCATTTAGATGATCAATACATCGTGTTACTCGATTTGCCAAACGGAACAGATGAATTACGTGAG
TTATTTAAAGTAGGATTCCCAGCGCGAATTTATACGCTGTTTTATCAAGAAAATAACCATCTATTTAGTACAGTTCCTAC
GAGAGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.455

99.358

0.521

Multiple sequence alignment