Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   K6134_RS18300 Genome accession   NZ_CP082045
Coordinates   3756965..3757729 (+) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain MS21324     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3751965..3762729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6134_RS18280 (K6134_18165) acpT 3752919..3753506 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  K6134_RS18285 (K6134_18170) nikA 3753617..3755191 (+) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  K6134_RS18290 (K6134_18175) nikB 3755191..3756135 (+) 945 WP_000947074.1 nickel ABC transporter permease subunit NikB -
  K6134_RS18295 (K6134_18180) nikC 3756132..3756965 (+) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  K6134_RS18300 (K6134_18185) amiE 3756965..3757729 (+) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  K6134_RS18305 (K6134_18190) nikE 3757726..3758532 (+) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  K6134_RS18310 (K6134_18195) nikR 3758538..3758939 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  K6134_RS18315 (K6134_18200) - 3759138..3759884 (+) 747 WP_001332161.1 GntR family transcriptional regulator -
  K6134_RS18320 (K6134_18205) - 3759909..3760382 (+) 474 WP_001161647.1 PTS sugar transporter subunit IIA -
  K6134_RS18325 (K6134_18210) - 3760379..3760660 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  K6134_RS18330 (K6134_18215) - 3760737..3762095 (+) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=524792 K6134_RS18300 WP_001136232.1 3756965..3757729(+) (amiE) [Escherichia coli strain MS21324]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=524792 K6134_RS18300 WP_001136232.1 3756965..3757729(+) (amiE) [Escherichia coli strain MS21324]
ATGCCACAACAGATTGAGCTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGAATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCA
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398