Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   JHX98_RS00940 Genome accession   NZ_CP067010
Coordinates   147474..148427 (+) Length   317 a.a.
NCBI ID   WP_002987790.1    Uniprot ID   A0A5S4TCX5
Organism   Streptococcus pyogenes strain iGAS376     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 142474..153427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHX98_RS00900 (JHX98_00900) - 143301..143666 (+) 366 WP_002986560.1 DUF1033 family protein -
  JHX98_RS00905 (JHX98_00905) comYA 143759..144697 (+) 939 WP_011888534.1 competence type IV pilus ATPase ComGA Machinery gene
  JHX98_RS00910 (JHX98_00910) comYB 144633..145667 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  JHX98_RS00915 (JHX98_00915) comYC 145669..145995 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  JHX98_RS00920 (JHX98_00920) comGD 145970..146398 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  JHX98_RS00925 (JHX98_00925) comGE 146355..146639 (+) 285 WP_041174183.1 competence type IV pilus minor pilin ComGE -
  JHX98_RS00930 (JHX98_00930) comYF 146632..147066 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  JHX98_RS00935 (JHX98_00935) comGG 147050..147376 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  JHX98_RS00940 (JHX98_00940) comYH 147474..148427 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  JHX98_RS00945 (JHX98_00945) - 148486..149682 (+) 1197 WP_011888536.1 acetate kinase -
  JHX98_RS00950 (JHX98_00950) - 149869..150177 (+) 309 WP_011017251.1 hypothetical protein -
  JHX98_RS00955 (JHX98_00955) proC 150260..151030 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -
  JHX98_RS00960 (JHX98_00960) pepA 151078..152145 (-) 1068 WP_011184093.1 glutamyl aminopeptidase -
  JHX98_RS00965 (JHX98_00965) - 152610..152894 (+) 285 WP_011888537.1 DUF4651 domain-containing protein -
  JHX98_RS00970 (JHX98_00970) - 152891..153208 (+) 318 WP_011888538.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35985.08 Da        Isoelectric Point: 4.7205

>NTDB_id=524319 JHX98_RS00940 WP_002987790.1 147474..148427(+) (comYH) [Streptococcus pyogenes strain iGAS376]
MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEGASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQT
EQLQANHQFTPDAIGFILLYLLEQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSLKYLKKDGFAIFLAPVNLLTSPQ
SQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIVLQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=524319 JHX98_RS00940 WP_002987790.1 147474..148427(+) (comYH) [Streptococcus pyogenes strain iGAS376]
ATGACTTTTGAAAAAATTGAAGAAGCTTATCAGCTGCTCTTAGAGAACTGTCAACTAATTGAAAATGATCTAAAAACACA
TATATACGATGCCATTGTTGAACAAAACTCCTTTTATTTAGGGGCTGAGGGAGCTAGTCCTCAAGTTGCTCAAAATAGTG
ATAAACTGAAAGCCTTGTGCCTGACAAAAGAAGAATGGCGTAAGGCCTACCAGTTTCTTTTTATTAAGGCAGCTCAGACT
GAGCAACTCCAAGCCAACCATCAGTTCACACCAGATGCTATTGGCTTCATTCTGCTGTATCTTTTGGAACAATTGAGTGA
TAAAGATAGCTTAGAGGTACTTGAGATTGGAAGTGGAACAGGGAACCTAGCCCAAACCCTTCTCAACAACACGAGCAAGA
GCCTTGATTATGTAGGGATTGAACTTGATGATCTCTTGATTGATCTGTCAGCCAGTATTGCTGAGATAATGGATTCTTCA
GCCCATTTTATTCAAGAAGATGCGGTAAGGCCTCAATTACTAAAAGAAAGTGACATTGTCATCAGTGACTTACCAGTTGG
TTATTATCCTAACGATGATATTGCCAAACGGTACAAGGTGGCTAGTTCAGATAAGCATACCTATGCCCATCATTTATTAA
TGGAACAGTCTTTAAAATACTTGAAAAAAGACGGTTTTGCGATTTTTCTGGCACCAGTCAATTTATTGACGAGCCCTCAG
AGCCAGTTATTGAAACAGTGGTTAAAAGATTATGCTCAGGTGGTGGCCTTGATTACGCTACCAGATTCTATTTTTGGTCA
TCCCTCTAATGCTAAGTCCATTATTGTCTTACAAAAACAAACAGACCACCCAATGGAAACCTTTGTCTATCCAATTCGGG
ATTTGAAGCTTGCAGAGAATATTCATGATTTTATGGAAAATTTCAAAAAATGGAAACTGAGTAATGTCAATTAA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(68-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TCX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

66.139

99.685

0.659
  comYH Streptococcus mutans UA140

65.823

99.685

0.656