Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   K3176_RS32670 Genome accession   NZ_CP081202
Coordinates   6838196..6838621 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain P9W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6833196..6843621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3176_RS32650 (K3176_32650) ileS 6833353..6836184 (+) 2832 WP_023103515.1 isoleucine--tRNA ligase -
  K3176_RS32655 (K3176_32655) lspA 6836177..6836686 (+) 510 WP_003094728.1 signal peptidase II -
  K3176_RS32660 (K3176_32660) fkpB 6836679..6837119 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  K3176_RS32665 (K3176_32665) ispH 6837205..6838149 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  K3176_RS32670 (K3176_32670) comF 6838196..6838621 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  K3176_RS32675 (K3176_32675) pilY2 6838618..6838965 (-) 348 WP_003161774.1 type 4a fimbrial biogenesis protein PilY2 -
  K3176_RS32680 (K3176_32680) pilY1 6838967..6842458 (-) 3492 WP_181268808.1 type 4a pilus biogenesis protein PilY1 -
  K3176_RS32685 (K3176_32685) pilX 6842470..6843057 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=521925 K3176_RS32670 WP_003094721.1 6838196..6838621(-) (comF) [Pseudomonas aeruginosa strain P9W]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=521925 K3176_RS32670 WP_003094721.1 6838196..6838621(-) (comF) [Pseudomonas aeruginosa strain P9W]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383