Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   K3831_RS17385 Genome accession   NZ_CP081008
Coordinates   3609106..3609870 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain XL10-gold-ultracompetent     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3604106..3614870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3831_RS17365 (K3831_17365) acpT 3605060..3605647 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  K3831_RS17370 (K3831_17370) nikA 3605758..3607332 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  K3831_RS17375 (K3831_17375) nikB 3607332..3608276 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  K3831_RS17380 (K3831_17380) nikC 3608273..3609106 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  K3831_RS17385 (K3831_17385) amiE 3609106..3609870 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  K3831_RS17390 (K3831_17390) nikE 3609867..3610673 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  K3831_RS17395 (K3831_17395) nikR 3610679..3611080 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  K3831_RS23715 - 3611283..3611489 (+) 207 Protein_3446 hypothetical protein -
  K3831_RS23170 - 3611491..3611613 (+) 123 Protein_3447 hypothetical protein -
  K3831_RS23175 - 3611825..3611959 (-) 135 WP_000660586.1 hypothetical protein -
  K3831_RS17400 (K3831_17400) metF 3612035..3612925 (+) 891 WP_000007523.1 methylenetetrahydrofolate reductase -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=521576 K3831_RS17385 WP_001136229.1 3609106..3609870(+) (amiE) [Escherichia coli strain XL10-gold-ultracompetent]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=521576 K3831_RS17385 WP_001136229.1 3609106..3609870(+) (amiE) [Escherichia coli strain XL10-gold-ultracompetent]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398