Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   K3F56_RS19575 Genome accession   NZ_CP080620
Coordinates   4097720..4098484 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain TOP10     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4092720..4103484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3F56_RS19555 (K3F56_19555) acpT 4093674..4094261 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  K3F56_RS19560 (K3F56_19560) nikA 4094372..4095946 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  K3F56_RS19565 (K3F56_19565) nikB 4095946..4096890 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  K3F56_RS19570 (K3F56_19570) nikC 4096887..4097720 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  K3F56_RS19575 (K3F56_19575) amiE 4097720..4098484 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  K3F56_RS19580 (K3F56_19580) nikE 4098481..4099287 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  K3F56_RS19585 (K3F56_19585) nikR 4099293..4099694 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  K3F56_RS22140 - 4099897..4100109 (+) 213 Protein_3867 hypothetical protein -
  K3F56_RS22425 - 4100080..4100154 (-) 75 Protein_3868 IS1 family transposase -
  K3F56_RS19595 (K3F56_19595) fimB 4100700..4101302 (-) 603 WP_000790589.1 type 1 fimbria switch DNA invertase FimB -
  K3F56_RS19600 (K3F56_19600) nanC 4102758..4103474 (+) 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=521179 K3F56_RS19575 WP_001136229.1 4097720..4098484(+) (amiE) [Escherichia coli strain TOP10]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=521179 K3F56_RS19575 WP_001136229.1 4097720..4098484(+) (amiE) [Escherichia coli strain TOP10]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398