Detailed information NTG0051938

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PADK2_RS02010
• Genome accession: NC_018080
• Coordinates: 431788..432936 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa DK2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 426788..437936

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PADK2_RS01985	-	427939..428235 (-)	297	WP_031655578.1	DUF167 domain-containing protein	-
PADK2_RS01990 (PADK2_01975)	-	428232..428825 (-)	594	WP_003084544.1	YggT family protein	-
PADK2_RS01995 (PADK2_01980)	proC	428836..429657 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PADK2_RS02000 (PADK2_01985)	-	429669..430361 (-)	693	WP_003117895.1	YggS family pyridoxal phosphate-dependent enzyme	-
PADK2_RS02005 (PADK2_01990)	pilT	430576..431610 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PADK2_RS02010 (PADK2_01995)	pilU	431788..432936 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PADK2_RS02015 (PADK2_02000)	czcD	432944..433843 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PADK2_RS02020 (PADK2_02005)	-	433997..434401 (+)	405	WP_003084560.1	NINE protein	-
PADK2_RS02025 (PADK2_02010)	-	434644..436017 (+)	1374	WP_003117896.1	pyridoxal-phosphate dependent enzyme	-
PADK2_RS02030 (PADK2_02015)	-	436014..437198 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=51938 PADK2_RS02010 WP_003100959.1 431788..432936(+) (pilU) [Pseudomonas aeruginosa DK2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=51938 PADK2_RS02010 WP_003100959.1 431788..432936(+) (pilU) [Pseudomonas aeruginosa DK2]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382