Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KZ312_RS25370 Genome accession   NZ_CP080260
Coordinates   5270527..5271291 (+) Length   254 a.a.
NCBI ID   WP_001136250.1    Uniprot ID   -
Organism   Escherichia coli strain 135     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 5265527..5276291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZ312_RS25350 (KZ312_25350) acpT 5266481..5267068 (+) 588 WP_000285765.1 4'-phosphopantetheinyl transferase AcpT -
  KZ312_RS25355 (KZ312_25355) nikA 5267179..5268753 (+) 1575 WP_000493130.1 nickel ABC transporter substrate-binding protein -
  KZ312_RS25360 (KZ312_25360) nikB 5268753..5269697 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  KZ312_RS25365 (KZ312_25365) nikC 5269694..5270527 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  KZ312_RS25370 (KZ312_25370) amiE 5270527..5271291 (+) 765 WP_001136250.1 nickel import ATP-binding protein NikD Regulator
  KZ312_RS25375 (KZ312_25375) nikE 5271288..5272094 (+) 807 WP_000173693.1 nickel import ATP-binding protein NikE -
  KZ312_RS25380 (KZ312_25380) nikR 5272099..5272500 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  KZ312_RS25385 (KZ312_25385) - 5272699..5273445 (+) 747 WP_021564691.1 GntR family transcriptional regulator -
  KZ312_RS25390 (KZ312_25390) - 5273470..5273943 (+) 474 WP_001377099.1 PTS sugar transporter subunit IIA -
  KZ312_RS25395 (KZ312_25395) - 5273940..5274221 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  KZ312_RS25400 (KZ312_25400) - 5274298..5275656 (+) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=518914 KZ312_RS25370 WP_001136250.1 5270527..5271291(+) (amiE) [Escherichia coli strain 135]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGSSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAALEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=518914 KZ312_RS25370 WP_001136250.1 5270527..5271291(+) (amiE) [Escherichia coli strain 135]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAACCGCTGGTGCACGGCGTATCGTTAACTCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCAGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCCGCACTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTTCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCTTTAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398