Detailed information NTG0518540

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: JD965_RS18260
• Genome accession: NZ_CP066219
• Coordinates: 3584274..3585419 (+)
• Length: 381 a.a.
• NCBI ID: WP_047475888.1
• Uniprot ID: -
• Organism: Bacillus siamensis strain B28
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3579274..3590419

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JD965_RS18245 (JD965_18245)	argS	3580022..3581692 (-)	1671	WP_016937597.1	arginine--tRNA ligase	-
JD965_RS18250 (JD965_18250)	-	3581689..3582117 (-)	429	WP_045926590.1	DUF1934 domain-containing protein	-
JD965_RS20420	-	3582271..3582642 (+)	372	Protein_3575	bacteriocin biosynthesis protein AlbG	-
JD965_RS18255 (JD965_18255)	-	3582649..3584043 (-)	1395	WP_047475887.1	YncE family protein	-
JD965_RS18260 (JD965_18260)	rapF	3584274..3585419 (+)	1146	WP_047475888.1	tetratricopeptide repeat protein	Regulator
JD965_RS18265 (JD965_18265)	-	3585403..3585522 (+)	120	WP_047475889.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
JD965_RS18270 (JD965_18270)	-	3585715..3585912 (+)	198	WP_198698193.1	helix-turn-helix transcriptional regulator	-
JD965_RS18275 (JD965_18275)	-	3585931..3586134 (+)	204	WP_045926595.1	hypothetical protein	-
JD965_RS18280 (JD965_18280)	-	3586169..3586354 (+)	186	WP_047475890.1	DUF4177 domain-containing protein	-
JD965_RS18285 (JD965_18285)	speB	3586826..3587698 (-)	873	WP_099743984.1	agmatinase	-
JD965_RS18290 (JD965_18290)	speE	3587757..3588587 (-)	831	WP_013353957.1	spermidine synthase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45320.40 Da
• Isoelectric Point: 4.9264

>NTDB_id=518540 JD965_RS18260 WP_047475888.1 3584274..3585419(+) (rapF) [Bacillus siamensis strain B28]
MASVVSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELASLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKQYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLITQIHYKLERYDKALEYHKRGVICSEESADSVYLHEFEFLKSLYINGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide

• Length: 1146 bp

>NTDB_id=518540 JD965_RS18260 WP_047475888.1 3584274..3585419(+) (rapF) [Bacillus siamensis strain B28]
GTGGCGAGTGTTGTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAAGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGTCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGCCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGTTGGATCAGAGGGAATATCTGTCAGCTATCAAGTTTTTTAAAAAAGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAGGTTTACTATTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCTTCATACGATATCAGGCTCATTCAATGCCAATCCTT
ATTCGGTGCAAATTTCCTGGATTTAAAACAATATGATCGGGCGATTACTCACTTTCAAAAGGCTTTTTCTTTAGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCCGGACACTTTATAATATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCAATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTCTGCCCCAAGCATATTTTTT
AATTACACAGATTCATTATAAATTGGAAAGATACGACAAAGCGCTCGAGTATCATAAAAGAGGCGTGATTTGTTCTGAAG
AGTCAGCAGATTCTGTTTATTTGCATGAGTTTGAATTTTTAAAATCTCTTTATATAAACGGACCTGATGAGGATGCCATG
AAACGAAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCTGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTACAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

• Tetratricopeptide repeat (TPR_12) (223-287)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	74.541	100	0.745
rapC	Bacillus subtilis subsp. subtilis str. 168	53.421	99.738	0.533