Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KZW90_RS23135 Genome accession   NZ_CP080233
Coordinates   4731450..4732214 (+) Length   254 a.a.
NCBI ID   WP_063085067.1    Uniprot ID   -
Organism   Escherichia coli O139:H1 strain P15-25     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4726450..4737214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZW90_RS23115 (KZW90_23115) acpT 4727404..4727991 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  KZW90_RS23120 (KZW90_23120) nikA 4728102..4729676 (+) 1575 WP_063085068.1 nickel ABC transporter substrate-binding protein -
  KZW90_RS23125 (KZW90_23125) nikB 4729676..4730620 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  KZW90_RS23130 (KZW90_23130) nikC 4730617..4731450 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  KZW90_RS23135 (KZW90_23135) amiE 4731450..4732214 (+) 765 WP_063085067.1 nickel import ATP-binding protein NikD Regulator
  KZW90_RS23140 (KZW90_23140) nikE 4732211..4733017 (+) 807 WP_000173690.1 nickel import ATP-binding protein NikE -
  KZW90_RS23145 (KZW90_23145) nikR 4733022..4733423 (+) 402 WP_053888681.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26804.42 Da        Isoelectric Point: 6.5997

>NTDB_id=518314 KZW90_RS23135 WP_063085067.1 4731450..4732214(+) (amiE) [Escherichia coli O139:H1 strain P15-25]
MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMQTHARETCLALGKPADDATLTAALEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=518314 KZW90_RS23135 WP_063085067.1 4731450..4732214(+) (amiE) [Escherichia coli O139:H1 strain P15-25]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCAGACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCTTTAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402