Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   JC772_RS12880 Genome accession   NZ_CP066187
Coordinates   2520368..2523880 (+) Length   1170 a.a.
NCBI ID   WP_048722281.1    Uniprot ID   A0AAX2CDJ0
Organism   Bacillus cytotoxicus strain E28.3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2515368..2528880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC772_RS12855 (JC772_12855) aceA 2515952..2517229 (+) 1278 WP_011983943.1 isocitrate lyase -
  JC772_RS12860 (JC772_12860) - 2517560..2517763 (+) 204 WP_011983944.1 cold shock domain-containing protein -
  JC772_RS12865 (JC772_12865) - 2518161..2518751 (+) 591 WP_011983945.1 competence protein ComK -
  JC772_RS22600 - 2518830..2518958 (-) 129 WP_275425714.1 hypothetical protein -
  JC772_RS12870 (JC772_12870) - 2519024..2519617 (+) 594 WP_011983946.1 TVP38/TMEM64 family protein -
  JC772_RS12875 (JC772_12875) lepB 2519678..2520244 (+) 567 WP_011983947.1 signal peptidase I -
  JC772_RS12880 (JC772_12880) addB 2520368..2523880 (+) 3513 WP_048722281.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  JC772_RS12885 (JC772_12885) addA 2523880..2527605 (+) 3726 WP_048723054.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  JC772_RS12890 (JC772_12890) - 2527698..2527913 (-) 216 WP_011983950.1 spore germination protein -
  JC772_RS12895 (JC772_12895) - 2527978..2528346 (-) 369 WP_011983951.1 spore germination protein GerPE -
  JC772_RS12900 (JC772_12900) - 2528361..2528555 (-) 195 WP_011983952.1 hypothetical protein -

Sequence

Protein


Download         Length: 1170 a.a.        Molecular weight: 134531.07 Da        Isoelectric Point: 5.3403

>NTDB_id=518307 JC772_RS12880 WP_048722281.1 2520368..2523880(+) (addB) [Bacillus cytotoxicus strain E28.3]
MSLRFVIGRAGSGKSTLCLREVQEELKERPRGKTILYLVPEQMTFQTQQALIGSEEVRASIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIIESRKEGLYVFQKAAEQNGFFEHLGGMIAEFKRYNITPSHVYEMWQQLDAHSSSAEQKLLA
NKIYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLPFSEYVKDAEIYIDGFHSFSPQELEIIRQLMRCGARMTITLTVDE
KTFAQPVNELDLFYETVLTYEKVKQVAREEKVVMEKTIPLVEQPRFHSPALAHLEAHYESRPNEKFYGETSIAIHTAANL
RAEVEGVAREIRRLVAEENYRYRDIAVLLRNGESYYDVLRTLFADYDIPHFIDEKRSMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVKKEVMREEMDEFENYCLAYGVQGKKWTADEPWTYRRYRSLDEGNEVITDRERQMEEKMNRLRE
IVRTPIIRLQKRLKRASTVMQMCEAVYLFLEELDVPKKLEQLRVRAEESGDFLFATDHDQVWEEVMNLLDTFVEMLGEES
MSLSVFIDVMTTGLEALQFANIPPSLDQVLIANIDRSRLSNIRATFVIGANEGVMPATPTDEGMLSDEERDVLAAAGVEL
APTTRQTLLEEQFVLYQMVTRASEKLYISCPLADEEGKTLLASSFMKKIKRMFPNVKETFVTNDVNDLSRAAQISYVTTP
EVTLSYVMQQLQTWKRYGFEGDLDFWWDVYNFYVTSDEWKQKSNRVLSSLFYRNRAKKLSTAVSRILYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLREKRTWADLSIKECEHLSILVIEEIAPLLQRQIL
LSSNRHAYLKQKLQQIIFRTSLVLREHAKASGFVPVDLEVPFGMGGKDSLPPMEFTLSNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVISNAGTWMKQGEVASPAGVLYFHIHNPLVEVKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSNIASEQEFSVLQKYVHHTFETIGKDITEGVIDIAP
YKMGNKAACTFCHFRSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGE

Nucleotide


Download         Length: 3513 bp        

>NTDB_id=518307 JC772_RS12880 WP_048722281.1 2520368..2523880(+) (addB) [Bacillus cytotoxicus strain E28.3]
ATGTCACTTCGATTTGTGATTGGAAGAGCTGGAAGTGGGAAAAGTACACTTTGTTTACGAGAAGTGCAAGAAGAGTTAAA
AGAGCGCCCAAGAGGGAAAACAATCTTGTATCTTGTTCCTGAACAAATGACATTTCAGACGCAGCAGGCGTTAATAGGAA
GTGAAGAGGTAAGAGCTTCTATTCGTGCACAAGTGTTTAGTTTTTCACGATTAGCTTGGAAAGTGTTGCAAGAAGTAGGA
GGGGCAAGTCGTCTTCATATTGATGAAGCGGGTGTGCATATGTTACTTCGCAAAATTATTGAATCGCGCAAAGAAGGATT
ATATGTGTTTCAAAAAGCCGCTGAACAAAATGGCTTCTTTGAACATCTTGGAGGTATGATTGCAGAGTTTAAACGTTACA
ATATAACGCCATCTCATGTATATGAAATGTGGCAACAGTTAGATGCGCATAGCAGTAGCGCCGAACAAAAGTTGCTGGCA
AATAAAATATATGATTTGCAGCTATTATATGATGATTTTGAACGAGCTTTAATTGGAAAGTATTTAGATTCGGAAGATTA
TTTACAGCTCCTTATTGAGAAACTGCCGTTTTCTGAGTATGTAAAGGATGCAGAAATTTATATTGATGGATTTCATTCGT
TCTCCCCGCAAGAGCTTGAAATTATAAGGCAGCTAATGAGATGCGGAGCAAGAATGACGATTACATTAACGGTGGATGAA
AAAACGTTCGCCCAGCCAGTCAATGAACTGGATTTGTTTTATGAAACAGTGCTGACGTATGAAAAAGTAAAACAAGTAGC
ACGTGAAGAGAAAGTGGTAATGGAAAAAACGATTCCGCTCGTAGAGCAGCCGCGATTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCACTATGAATCGCGTCCGAACGAAAAGTTTTACGGAGAGACGAGTATTGCCATCCATACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGAGTAGCGCGAGAAATTCGTAGACTTGTTGCAGAAGAGAATTATCGATACCGCGATATTGCCGT
ACTTCTTCGGAACGGAGAAAGCTATTATGATGTGTTAAGAACATTATTTGCAGATTATGATATTCCGCATTTCATTGATG
AAAAGCGCTCGATGTCTCACCATCCGTTAGTCGAATGCATTCGTTCTGCTTTAGAAATAATTAGTGGGAATTGGCGTTAT
GATGCTGTTTTTCGCTGCGTGAAAACAGAACTTTTATATCCGCTGGATGTAAAAAAAGAAGTGATGCGTGAAGAAATGGA
TGAGTTTGAAAACTACTGTTTGGCGTATGGTGTCCAAGGTAAGAAATGGACCGCCGATGAGCCGTGGACGTATCGTCGTT
ATCGCTCTCTTGATGAGGGGAACGAGGTGATAACAGATCGTGAACGCCAAATGGAAGAGAAAATGAACCGTCTTCGTGAG
ATTGTAAGAACGCCAATCATTCGTCTGCAAAAAAGATTGAAACGTGCAAGTACTGTTATGCAAATGTGCGAAGCTGTATA
TTTATTTTTAGAGGAGCTAGATGTTCCGAAGAAATTAGAACAACTACGTGTACGTGCTGAAGAAAGCGGGGATTTCTTAT
TTGCGACAGACCATGATCAAGTGTGGGAAGAAGTGATGAATCTTCTTGATACATTTGTTGAGATGCTTGGAGAAGAAAGT
ATGTCTCTATCGGTGTTTATTGATGTGATGACGACAGGGCTTGAGGCACTTCAATTTGCCAATATCCCGCCATCGTTAGA
CCAAGTATTAATTGCAAATATCGATCGCTCTAGACTTTCAAATATTCGGGCAACATTTGTGATTGGTGCAAATGAAGGTG
TGATGCCAGCTACGCCGACAGATGAAGGGATGCTTTCTGATGAAGAAAGAGATGTTCTTGCAGCAGCGGGCGTGGAATTA
GCACCAACGACAAGACAAACGTTATTAGAAGAACAGTTTGTCTTGTATCAAATGGTGACAAGAGCATCTGAGAAACTATA
TATCTCTTGTCCTCTTGCAGATGAAGAAGGAAAGACATTACTAGCTTCTAGTTTTATGAAGAAAATAAAGAGAATGTTTC
CTAATGTGAAAGAGACGTTTGTAACAAACGATGTAAACGACTTATCGCGCGCAGCACAAATTTCATATGTAACGACGCCA
GAAGTAACATTGTCATATGTGATGCAACAGCTGCAAACGTGGAAGCGATACGGTTTTGAAGGTGATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTTACATCCGATGAATGGAAGCAAAAAAGCAACCGTGTATTATCCAGTTTGTTTTATCGAA
ATCGTGCGAAGAAATTAAGTACGGCTGTAAGTCGAATTTTATATGGAGATAAAATAAAAGGAAGTGTCTCTCGTATGGAA
TTATTTAATCGCTGTGCGTACGCTCATTTTGCACAGCACGGTTTATCATTGAGAGAGCGCGATATATTTAAATTAGATGC
ACCAGATATCGGGGAGCTGTTCCATGCAGCGCTAAAGAAAATTGCGGACAAGCTCTTGCGTGAAAAACGGACTTGGGCTG
ATTTATCCATAAAAGAATGTGAGCATCTTTCCATATTAGTAATAGAAGAAATTGCTCCATTATTACAAAGGCAAATTTTA
TTAAGCTCAAATCGACATGCTTATTTGAAACAAAAATTACAGCAAATTATTTTTAGAACATCGCTTGTGCTTAGGGAACA
TGCTAAGGCGAGTGGATTTGTTCCAGTTGATTTAGAAGTTCCGTTTGGTATGGGAGGCAAAGACTCATTACCACCGATGG
AATTTACATTATCAAACGGTGTGAAAATGGAAGTTGTCGGCCGGATTGATCGAGTTGATAAAGCGGAAGATGAGAGCGGT
ACGTTTCTTCGCATTATTGATTATAAATCGAGCTCGAAAGCGCTGGATTTAACAGAAGTATATTACGGATTAGCATTGCA
AATGCTGACGTATTTGGATGTCGTCATTTCTAATGCTGGTACGTGGATGAAACAGGGGGAAGTCGCATCACCAGCAGGTG
TGTTATACTTCCATATTCATAATCCACTTGTTGAGGTGAAAGGTGACGCTTCTGAAGAGGAGATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTGCTTGGAGATGCAGATGTTGTTCGTTTAATGGATAACAAACTATCAACAGGAAG
CTCTGACATTATTTCAGCCGGGTTAAAAAAAGACGGAAGCTTCAGCGCACGCTCTAACATCGCAAGTGAACAAGAATTTT
CTGTGCTACAAAAATACGTACACCATACGTTTGAAACGATCGGAAAAGACATTACAGAAGGTGTCATTGATATTGCTCCA
TATAAAATGGGGAATAAAGCAGCGTGCACATTTTGTCACTTCCGCTCTGTCTGCCAGTTCGATGAATCGCTGGAAGACAA
TCAGTTCCGTACATTAAAAGATATGAAAGATAGTGAAGCAATGGAGAAAATACGAGAGGAGGTCGGGGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(307-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.53

100

0.496