Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   JC773_RS01245 Genome accession   NZ_CP066185
Coordinates   215300..217639 (-) Length   779 a.a.
NCBI ID   WP_087099239.1    Uniprot ID   -
Organism   Bacillus cytotoxicus strain PDT2.12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 210300..222639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC773_RS01215 (JC773_01215) - 210341..210517 (-) 177 WP_012095581.1 hypothetical protein -
  JC773_RS01220 (JC773_01220) - 210604..210795 (-) 192 WP_041810042.1 hypothetical protein -
  JC773_RS01225 (JC773_01225) - 211064..211753 (-) 690 WP_012095582.1 alpha/beta hydrolase -
  JC773_RS01230 (JC773_01230) dtd 211889..212329 (-) 441 WP_012095583.1 D-aminoacyl-tRNA deacylase -
  JC773_RS01235 (JC773_01235) relA 212347..214530 (-) 2184 WP_210351820.1 GTP diphosphokinase -
  JC773_RS01240 (JC773_01240) - 214739..215251 (-) 513 WP_012095585.1 adenine phosphoribosyltransferase -
  JC773_RS01245 (JC773_01245) recJ 215300..217639 (-) 2340 WP_087099239.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  JC773_RS01250 (JC773_01250) - 217719..218612 (-) 894 WP_012095587.1 cation diffusion facilitator family transporter -
  JC773_RS01255 (JC773_01255) secDF 218894..221152 (-) 2259 WP_012095588.1 protein translocase subunit SecDF -
  JC773_RS01260 (JC773_01260) - 221453..221734 (-) 282 WP_012095589.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88282.14 Da        Isoelectric Point: 4.8930

>NTDB_id=518229 JC773_RS01245 WP_087099239.1 215300..217639(-) (recJ) [Bacillus cytotoxicus strain PDT2.12]
MLQPKTRWKEKTYNEELVRELASKLQLSPLVVSLFLGRGLDTEDKIMDFFNTAEQEFHDPFLLEGMDRTVERVKQAIQNG
EQILIFGDYDADGVSSTTVLFLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHQAGFSLIITVDTGIAAVHEAQVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLI
VQRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFLLAPRINAVGRLEDAAPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIIEVECKYPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDSEKQIAKGSARSIEGFDLF
ANLSDCRDLLPHFGGHPMAAGMTLHMNDVDELRRRLNEQANAVLVEEDFIPITTIDAVCKVEDVTLSAIEDMQKLAPFGV
GNPKPCIAVKDADLESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVVGEVSINEWNNFKKPQLMMQ
DIAVEAWQLFDWRSMRNVEVNLAELPKEKIIIVYFSEDVLNKFSLEGYKEQLVHASAVTRLDGEYIVLLDLPKGIEELRN
IFQVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLHQKAPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMAEQIQKRDLTESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEIEQLR

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=518229 JC773_RS01245 WP_087099239.1 215300..217639(-) (recJ) [Bacillus cytotoxicus strain PDT2.12]
GTGTTACAACCGAAAACGCGTTGGAAAGAAAAAACATATAATGAAGAACTGGTGAGGGAATTAGCAAGTAAATTGCAATT
ATCACCACTTGTTGTTTCTTTATTCCTCGGTAGAGGTTTAGATACAGAAGATAAGATTATGGATTTTTTTAATACAGCAG
AGCAAGAATTTCATGATCCATTTTTATTAGAAGGCATGGATCGGACAGTAGAGCGTGTGAAACAGGCGATCCAAAATGGA
GAGCAGATTTTAATATTTGGTGACTATGATGCAGACGGAGTCAGTAGTACGACCGTACTATTTTTAGCGCTTCAAGAATT
AGGAGCAGATGTGGAATTTTATATTCCAAACCGTTTTACGGAAGGATATGGACCAAATGAAGAGGCGTTTCGTTGGGCGC
ACCAAGCCGGTTTTTCTTTAATTATTACGGTTGATACAGGAATAGCAGCAGTACATGAGGCGCAAGTGGCAAAAGAGCTT
GGAATTGATTTAATTATTACAGATCATCATGAGCCACCGCCAGAACTGCCAGAAGCACTTGCGATTATTCATCCGAAGCT
TGAGGGTGGTGTATATCCGTTTCACTATTTAGCAGGGGTTGGTGTTGCTTTTAAAGTAGCGCATGCGTTGCTAGGGCGTG
TTCCGGAGCATTTGCTAGAAATTGCTGTTATTGGTACAGTAGCTGACTTAGTATCACTTCATGGTGAGAATAGATTAATT
GTACAGCGCGGATTAAAACATATGAGAATGACAAAAAACATCGGCTTAAAAGCACTTTTTAAAGTGGCGAATGTCTCGCA
AAGTGAAATTACAGAAGAAAGTATTGGTTTTTTGTTGGCGCCTCGTATTAATGCGGTTGGACGTTTAGAAGATGCTGCTC
CGGCAGTTCATCTTCTGCTATCAGAAGATCCAGAAGAAGCAAAAGAGCTGGCAGAAGAAATTGATGAATTAAACAAACTA
CGAAAAGATATTGTGAAACAAATTACCGAGGAAGCAATTATTGAGGTGGAATGTAAATATCCACCAGAGGAGAATAAAGT
GCTTGTTCTTGCGAAAGAAGGCTGGAACCCGGGGGTAATTGGAATTGTTGCTTCTAAATTGGTAGAACGTTTTTATCGTC
CGACGATTGTGTTAAGTATTGATTCTGAGAAACAAATTGCCAAGGGATCTGCGCGCAGTATTGAAGGGTTTGATTTGTTT
GCTAATTTATCGGATTGTAGAGATTTATTACCTCATTTTGGAGGTCATCCAATGGCAGCAGGGATGACGCTTCATATGAA
TGATGTAGATGAACTGAGACGCCGTTTGAATGAACAAGCGAATGCAGTCTTAGTAGAGGAAGATTTTATTCCGATTACAA
CTATAGATGCCGTTTGTAAAGTAGAGGATGTCACTCTTTCAGCAATTGAAGATATGCAGAAGTTGGCACCATTTGGTGTT
GGAAATCCAAAACCATGTATCGCAGTGAAAGATGCTGATTTAGAAAGTATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGGGATGGACAAGCTACGCTAGATACAATTGGTTTTGGATTTGGTGCCTATGCAAAAGAGATTTCTC
CAGTTGCGAAAGTTTCTGTTGTAGGAGAAGTATCTATTAACGAATGGAATAATTTTAAGAAACCGCAGTTAATGATGCAA
GATATCGCAGTAGAAGCGTGGCAACTATTTGATTGGCGCAGCATGCGTAATGTAGAAGTGAATTTAGCAGAACTTCCAAA
AGAAAAAATAATTATAGTGTATTTTTCTGAAGATGTATTGAATAAATTTTCTTTAGAAGGTTATAAAGAGCAACTTGTGC
ATGCATCAGCAGTCACTCGTTTAGATGGTGAGTATATTGTTTTACTTGACTTACCAAAAGGGATAGAAGAGTTGCGAAAT
ATATTTCAAGTTGGCTTCCCGGCTCGTATTTATACGTTATTTTATCAAGAAAATAATCATTTGTTTAGCACAGTTCCAAC
GAGAGAACACTTTAAATGGTATTACTCTTTCTTACATCAAAAAGCCCCATTTTCTTTAAGGCAATATGGCGAGCAACTAT
GCCGTCATAAAGGGTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCCGAACAAATTCAAAAGCGTGATTTAACTGAATCGAACACATATCGTGAGAAAATGAACCA
TTTACAACTTGAAAAAGAATTAGTATATAGCACATATCAACAGCTGTACACATGGTTTGAAACGATTCGTAATCATAAAG
AAATAGAACAGTTAAGATAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.488

99.358

0.531