Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KZY08_RS05280 Genome accession   NZ_CP080163
Coordinates   1112493..1112960 (-) Length   155 a.a.
NCBI ID   WP_000338336.1    Uniprot ID   -
Organism   Escherichia coli strain PK8275     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1107493..1117960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZY08_RS05255 (KZY08_05260) rpsG 1107634..1108104 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KZY08_RS05260 (KZY08_05265) fusA 1108201..1110315 (+) 2115 WP_000124703.1 elongation factor G -
  KZY08_RS05265 (KZY08_05270) tuf 1110386..1111570 (+) 1185 WP_000031783.1 elongation factor Tu -
  KZY08_RS05270 (KZY08_05275) bfd 1111753..1111947 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KZY08_RS05275 (KZY08_05280) bfr 1112020..1112496 (+) 477 WP_000675515.1 bacterioferritin -
  KZY08_RS05280 (KZY08_05285) pilD 1112493..1112960 (-) 468 WP_000338336.1 prepilin peptidase Machinery gene
  KZY08_RS05285 (KZY08_05290) rpsJ 1113339..1113650 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KZY08_RS05290 (KZY08_05295) rplC 1113683..1114312 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KZY08_RS05295 (KZY08_05300) rplD 1114323..1114928 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KZY08_RS05300 (KZY08_05305) rplW 1114925..1115227 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KZY08_RS05305 (KZY08_05310) rplB 1115245..1116066 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KZY08_RS05310 (KZY08_05315) rpsS 1116083..1116361 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KZY08_RS05315 (KZY08_05320) rplV 1116376..1116708 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KZY08_RS05320 (KZY08_05325) rpsC 1116726..1117427 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KZY08_RS05325 (KZY08_05330) rplP 1117440..1117850 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=517933 KZY08_RS05280 WP_000338336.1 1112493..1112960(-) (pilD) [Escherichia coli strain PK8275]
MDAALPFLILYACLSTLLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=517933 KZY08_RS05280 WP_000338336.1 1112493..1112960(-) (pilD) [Escherichia coli strain PK8275]
ATGGACGCTGCCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAACCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGGTTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTTTGCTTATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGTCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368