Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KZX76_RS04950 Genome accession   NZ_CP080119
Coordinates   1014207..1014971 (+) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain M45     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1009207..1019971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZX76_RS04930 (KZX76_04930) acpT 1010161..1010748 (+) 588 WP_000285760.1 4'-phosphopantetheinyl transferase AcpT -
  KZX76_RS04935 (KZX76_04935) nikA 1010859..1012433 (+) 1575 WP_000493124.1 nickel ABC transporter substrate-binding protein -
  KZX76_RS04940 (KZX76_04940) nikB 1012433..1013377 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  KZX76_RS04945 (KZX76_04945) nikC 1013374..1014207 (+) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  KZX76_RS04950 (KZX76_04950) amiE 1014207..1014971 (+) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  KZX76_RS04955 (KZX76_04955) nikE 1014968..1015774 (+) 807 WP_000173684.1 nickel import ATP-binding protein NikE -
  KZX76_RS04960 (KZX76_04960) nikR 1015780..1016181 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  KZX76_RS04965 (KZX76_04965) - 1016380..1017126 (+) 747 WP_001296489.1 GntR family transcriptional regulator -
  KZX76_RS04970 (KZX76_04970) - 1017151..1017624 (+) 474 WP_001161648.1 PTS sugar transporter subunit IIA -
  KZX76_RS04975 (KZX76_04975) - 1017621..1017902 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  KZX76_RS04980 (KZX76_04980) - 1017979..1019337 (+) 1359 WP_001525857.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=517264 KZX76_RS04950 WP_001136232.1 1014207..1014971(+) (amiE) [Escherichia coli strain M45]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=517264 KZX76_RS04950 WP_001136232.1 1014207..1014971(+) (amiE) [Escherichia coli strain M45]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAAGCACGCATCCTCGATCTACTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTAGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCGCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398