Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KXJ73_RS01320 Genome accession   NZ_CP079747
Coordinates   292372..293136 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain DL21     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 287372..298136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KXJ73_RS01310 (KXJ73_01305) nikR 291162..291563 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  KXJ73_RS01315 (KXJ73_01310) nikE 291569..292375 (-) 807 WP_000173665.1 nickel import ATP-binding protein NikE -
  KXJ73_RS01320 (KXJ73_01315) amiE 292372..293136 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  KXJ73_RS01325 (KXJ73_01320) nikC 293136..293969 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  KXJ73_RS01330 (KXJ73_01325) nikB 293966..294910 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  KXJ73_RS01335 (KXJ73_01330) nikA 294910..296484 (-) 1575 WP_000953348.1 nickel ABC transporter substrate-binding protein -
  KXJ73_RS01340 (KXJ73_01335) acpT 296595..297182 (-) 588 WP_000285773.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=514917 KXJ73_RS01320 WP_001136236.1 292372..293136(-) (amiE) [Escherichia coli strain DL21]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=514917 KXJ73_RS01320 WP_001136236.1 292372..293136(-) (amiE) [Escherichia coli strain DL21]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398